Advertisement
Research Article

Contribution of Noncentrosomal Microtubules to Spindle Assembly in Drosophila Spermatocytes

  • Elena Rebollo,

    Affiliation: Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, Heidelberg, Germany

    X
  • Salud Llamazares,

    Affiliation: Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, Heidelberg, Germany

    X
  • José Reina,

    Affiliation: Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, Heidelberg, Germany

    X
  • Cayetano Gonzalez mail

    To whom correspondence should be addressed. E-mail: cgonzalez@cnio.es

    Affiliation: Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, Heidelberg, Germany

    X
  • Published: January 20, 2004
  • DOI: 10.1371/journal.pbio.0020008

Abstract

Previous data suggested that anastral spindles, morphologically similar to those found in oocytes, can assemble in a centrosome-independent manner in cells that contain centrosomes. It is assumed that the microtubules that build these acentrosomal spindles originate over the chromatin. However, the actual processes of centrosome-independent microtubule nucleation, polymerisation, and sorting have not been documented in centrosome-containing cells. We have identified two experimental conditions in which centrosomes are kept close to the plasma membrane, away from the nuclear region, throughout meiosis I in Drosophila spermatocytes. Time-lapse confocal microscopy of these cells labelled with fluorescent chimeras reveals centrosome-independent microtubule nucleation, growth, and sorting into a bipolar spindle array over the nuclear region, away from the asters. The onset of noncentrosomal microtubule nucleation is significantly delayed with respect to nuclear envelope breakdown and coincides with the end of chromosome condensation. It takes place in foci that are close to the membranes that ensheath the nuclear region, not over the condensed chromosomes. Metaphase plates are formed in these spindles, and, in a fraction of them, some degree of polewards chromosome segregation takes place. In these cells that contain both membrane-bound asters and an anastral spindle, the orientation of the cytokinesis furrow correlates with the position of the asters and is independent of the orientation of the spindle. We conclude that the fenestrated nuclear envelope may significantly contribute to the normal process of spindle assembly in Drosophila spermatocytes. We also conclude that the anastral spindles that we have observed are not likely to provide a robust back-up able to ensure successful cell division. We propose that these anastral microtubule arrays could be a constitutive component of wild-type spindles, normally masked by the abundance of centrosome-derived microtubules and revealed when asters are kept away. These observations are consistent with a model in which centrosomal and noncentrosomal microtubules contribute to the assembly and are required for the robustness of the cell division spindle in cells that contain centrosomes.

Introduction

Two different pathways of spindle assembly are known to operate in the animal kingdom. The first, observed in somatic as well as in male germline cells, requires the microtubule organising activity of centrosomes (Compton 2000; Bornens 2002). The second, restricted to female germline and some embryonic cells that lack centrosomes, is thought to depend upon the microtubule stabilisation and organisation activity of the chromosomes themselves (McKim and Hawley 1995; de Saint Phalle and Sullivan 1998; reviewed in Karsenti and Vernos 2001). Centrosome-independent microtubule growth and sorting into a bipolar spindle have been observed in vitro around chromatin-coated beads in Xenopus egg extracts (Heald et al. 1996). Moreover, some experimental data suggest that a centrosome-independent pathway for spindle assembly also exists in somatic cells (Bonaccorsi et al. 1998, 2000; Megraw et al. 1999, 2001; Vaizel-Ohayon and Schejter 1999; Khodjakov et al. 2000; Hinchcliffe et al. 2001; reviewed in Raff 2001). It is generally assumed that the microtubules that build these acentrosomal spindles originate over the chromatin. However, so far, the actual process of centrosome-indepen dent microtubule nucleation, polymerisation, and sorting into a bipolar spindle has not been documented in any of the centrosome-containing cell lineages of a living animal.

The problem in visualising such microtubules of noncentrosomal origin when centrosomes are present is a technical one. In Drosophila, as in most animal cells, at the onset of cell division the two segregated pairs of centrosomes have a strong microtubule organising activity (Tates 1971; Church and Lin 1982; Cenci et al. 1994). Consequently, as soon as the nuclear envelope (NE) breaks down, numerous microtubules invade the nuclear region, making it extremely difficult to single out any noncentrosomal microtubules that might be present. To circumvent this limitation, we have taken advantage of two experimental conditions that inhibit the natural process of centriole migration from the plasma membrane to the interior of the cell that takes place at the onset of meiosis in Drosophila spermatocytes. Under such conditions, the centrosomes organise asters, but these are kept at the plasma membrane, away from the nuclear region. In these cells, microtubules can be seen to grow from the remnants of the fenestrated NE and to assemble into anastral bipolar spindles in a centrosome-independent manner. We propose that these spindle-shaped arrays correspond to a subset of microtubules that are normally present in the spindles of wild-type cells.

Results

Centriole Migration towards the Nucleus in Drosophila Spermatocytes Requires Microtubules and the Function of asp

Studies based on electron microscopy (Tates 1971) had shown that soon after the last round of mitotic divisions that precede meiosis in Drosophila spermatocytes, the centrioles migrate towards the periphery of the cell and position themselves underneath the plasma membrane. The same studies revealed that shortly before the onset of prometaphase I, the centrioles are found again close to the nuclear membrane, thus strongly suggesting that they migrate back near the nucleus in preparation for meiosis. Using an endogenously expressed centriolar green fluorescent protein (GFP) marker, we have been able to demonstrate such migration in living spermatocytes (Figure 1A; Video S1). The entire process takes about 2 h. Initially, the two centriolar pairs move towards the nucleus and start to migrate apart as they approach the nuclear membrane. They finally position themselves at opposite sides of the nucleus, about 30 min before the onset of NE breakdown (NEB).

thumbnail

Figure 1. Centriole Migration in Primary Spermatocytes

(A) Time-lapse series of confocal images from a wild-type primary spermatocyte expressing GFP-PACT (centrioles) and His2AvD–GFP (chromosomes). The centrioles (arrows) can be seen moving away from the plasma membrane (0) towards the nucleus (N) and then migrating diametrically apart as the chromatin condenses. The chromosomes are fully condensed at timepoint 121 min.

(B–D) The two centriole pairs (green) projected over the phase-contrast view (grey) can be seen close to the fenestrated NE and away from the plasma membrane (pm) in control cells (B), while they remain plasma membrane-bound in asp (C) and in colcemid-treated wild-type cells (D). In asp spermatocytes (C), the position of the membrane-bound centrioles correlates tightly with the pointed end of phase-dark protrusions (arrows) that are not present in colcemid-treated cells. These reflect the distribution of phase-contrast membranes known to overlap microtubules in these cells.

(E–J) XY projections (E–G) and their corresponding optical sections (H–J) of control (E and H), asp (F and I), colcemid-treated spermatocytes (G and J) expressing an endogenous GFP–α-tubulin confirm that the two major MTOCs in control cells are close to the nucleus, but remain near the plasma membrane in the two experimental conditions. MTOC activity in colcemid-treated spermatocytes was assayed following a 1-s pulse of 350 nm light to inactivate the drug, thus allowing microtubule regrowth. The yellow bar in the XY projections (E–G) marks the position of the corresponding XZ optical sections (H–J).

doi:10.1371/journal.pbio.0020008.g001

We have identified two experimental conditions that inhibit centriole movement, back from the plasma membrane in Drosophila spermatocytes. The first one is mutation in the gene abnormal spindle, asp (Ripoll et al. 1985; Casal et al. 1990; Gonzalez et al. 1990; Saunders et al. 1997; do Carmo Avides and Glover 1999; Wakefield et al. 2001; Riparbelli et al. 2002). In contrast to wild-type control cells (Figure 1B), the two pairs of centrioles in aspE3/aspL1 spermatocytes at late prophase are still located at the plasma membrane (Figure 1C), where they remain throughout meiosis. Centriole migration back towards the NE can also be inhibited by microtubule depolymerisation. Like in asp mutant spermatocytes, the centrioles of wild-type spermatocytes exposed to the microtubule-depolymerising drug colcemid remain close to the plasma membrane throughout meiosis (Figure 1D).

Top (Figure 1F) and lateral (Figure 1I) views of living asp mutant spermatocytes expressing a GFP–α-tubulin fusion reveal that the microtubule organising centres (MTOCs) are found at the periphery of these cells. The MTOCs of control cells at this stage can be seen near the nuclear membrane (Figure 1E and 1H). These observations strongly suggest that the membrane-bound centrioles observed in asp mutant spermatocytes are associated to active centrosomes that retain MTOC activity. This conclusion is further substantiated by the localisation of the pericentriolar material (PCM) marker γ-Tub23C (Zheng et al. 1991; Sunkel et al. 1995) around the membrane-bound centrioles, coinciding with the position of the MTOCs (data not shown). The same applies to cells in which centriole migration is inhibited by colcemid. Immediately after a short pulse of 350 nm UV light to inactivate the drug (W. E. Theurkauf, personal communication), two asters are organised around the membrane-bound centrioles in these cells (Figure 1G and 1J).

Anastral Spindles Are Assembled When the Centrosomes Are Kept Membrane Bound

Inhibition of centrosome migration back from the plasma membrane in Drosophila spermatocytes offers an unprecedented opportunity to assay centrosome-independent microtubule polymerisation during spindle assembly in the cells of a living animal that contain centrosomes. Therefore, we decided to follow microtubules by time-lapse confocal microscopy in asp and colcemid-treated cells that expressed a GFP–α-tubulin fusion, as they went through meiosis. At the onset of prometaphase, the NE becomes fenestrated, but does not disappear in Drosophila (Tates 1971; Stafstrom and Staehelin 1984; Church and Lin 1985). This partial NEB can be readily identified by the sudden entry of GFP–α-tubulin into the nuclear region (Figure 2, timepoint 0; Video S2). In control cells, microtubule polymerisation and organisation are largely concentrated around the centrosomes (Church and Lin 1982; Cenci et al. 1994). Consequently, the abundance of these microtubules makes it extremely difficult to determine the possible contribution of any centrosome-independent microtubule polymerisation activity to spindle assembly (Figure 2, control, 10 min to 32 min; Video S2). Microtubule organisation is significantly different in the case of asp mutant spermatocytes. At the time of NEB, the membrane-bound centrosomes can be seen organising the two asters at a significant distance from the nucleus, which is kept clear from astral microtubules (Figure 2; Video S3). Around 10 min after NEB, a distinct focus of microtubule polymerisation appears within the nuclear region, away from the asters (Figure 2, asp, 10 min; Video S3). It gives rise to a few bundles (Figures 2, 15 min) that grow (Figure 2, 22 min) and get organised into a bipolar spindle-shaped microtubule array that in 28% (n = 43) of the cells is anastral and establishes no contact with the membrane-bound centrosomes (Figure 2, 39 min). The remaining 72% was accounted for by cells in which, despite the distance, microtubules from one or both asters reach the spindle so that spindle poles and asters were aligned. Although the acentrosomal origin of the spindle microtubules in these cells is fairly convincing, only those cells that assembled truly anastral spindles that remained so throughout meiosis were considered as cases of noncentrosomal spindle assembly.

thumbnail

Figure 2. Time-Lapse Series of Meiosis Progression in Control, asp, and Colcemid-Treated Spermatocytes

Timepoint 0 coincides with the time of NEB revealed by the sudden entry of GFP signal into the nucleus. In control cells (Video S2), microtubules are mainly organised around the centrosomes (arrows). However, when centrosomes are kept away from the nuclear region by mutation in asp (Video S3) or colcemid treatment (Video S4), microtubule nucleation and growth are clearly revealed over the nuclear region (N), well away from the centrosomes. Such noncentrosomal microtubules may form bundles that eventually are sorted into spindlelike bipolar microtubule arrays. Microtubules were labelled with an endogenous GFP–α-tubulin fusion.

doi:10.1371/journal.pbio.0020008.g002

These observations strongly suggested that microtubules can nucleate in a centrosome-independent manner and assemble a spindle-like array in Drosophila spermatocytes. The question remained open, however, as to whether such anastral structures could not simply be a consequence of mutation in asp itself. To rule out such a possibility, we followed spindle assembly in wild-type spermatocytes in which centrosomes had been kept membrane-bound by colcemid treatment. The results were strikingly similar to those observed in asp cells. Seconds after colcemid inactivation, the cortex-bound position of the centrosomes is revealed by the growing asters that were not visible before (Figure 2, colcemid inactivation, 0 timepoint; Video S4). Like in asp mutant spermatocytes, microtubules can clearly be seen to nucleate over the nuclear region, well away from the asters (Figure 2, 11 min), grow (Figure 2, 15 min), and get sorted (Figure 2, 37 min and 43 min) into anastral bipolar arrays. Of the colcemid-treated cells studied (n = 32), 22% behaved like the cell shown in Figure 2. The remaining cells assembled more than one spindle, multipolar spindles, or spindles that were connected to one of the asters.

The Nucleation of Noncentrosomal Microtubules in Spermatocytes with Membrane-Bound Centrosomes Has a Late Onset

We then decided to time the onset of anastral microtubule nucleation. The timing of the main landmarks of meiosis progression in control, asp, and colcemid-treated spermatocytes is summarised in Figure 3. In control spermatocytes, chromosome condensation starts within 2 min after NEB, and the first centrosomal microtubules enter the nuclear region shortly afterwards. Chromosome condensation is completed between 10 and 12 min after NEB. In agreement with previous reports, anaphase onset takes place between 32 and 47 min after NEB (Church and Lin 1985; Rebollo and Gonzalez 2000; Savoian et al. 2000). Remarkably, the timing of meiosis progression from NEB to anaphase onset, both in asp and in colcemid-treated wild-type spermatocytes, seems to be largely unaffected, suggesting that the feeble spindle checkpoint of these cells (Rebollo and Gonzalez 2000; Savoian et al. 2000) is not triggered by the membrane-bound centrosomes' condition. The timing of the onset of noncentrosomal microtubule growth within the nuclear region in asp and colcemid-treated cells is tightly controlled. It occurs between 9 and 13 min after NEB, at the same time or marginally later than the end of chromosome condensation. If this process occurs with the same timing in wild-type cells, the noncentrosomal microtubules will intermingle with numerous centrosomal microtubules that are already present at this stage.

thumbnail

Figure 3. The Timing of Noncentrosomal Microtubule Nucleation

Referred to NEB (timepoint 0), the timing of chromosome condensation and of onset of chromosome segregation is essentially identical in control, asp, and colcemid-treated spermatocytes. In control spermatocytes, aster microtubules can be seen entering the nuclear region 3–6 min after NEB. They do not in asp or following colcemid treatment. In these two cases, however, centrosome-independent microtubule polymerisation can be seen over the nuclear region. It starts between 9 and 13 min after NEB, coinciding with or very shortly after the end of chromosome condensation.

doi:10.1371/journal.pbio.0020008.g003

Acentrosomal Microtubules Are Nucleated on the Inner Side of the Remnants of the NE and Not around the Chromosomes

To determine the nucleation site of the microtubules organised over the nuclear region, we followed the initial stages of microtubule assembly by time-lapse microscopy, acquiring several Z series of XY confocal and phase-contrast sections at different timepoints. From these, we generated a time-lapse series of 3D reconstructions that allowed us to localise the foci of nucleation of anastral microtubules. We were able to draw the following three main conclusions that apply to both asp and colcemid-treated spermatocytes. Firstly, the foci from which microtubules grow may be clustered (Figure 4A) or dispersed (Figure 4B). Secondly, no significant correlation can be established between the site of microtubule nucleation and the chromosomes (Figure 4A and 4B). Finally, nucleation takes place in close proximity to the remnants of the NE (ten out of ten cells reconstructed; Figure 4A and 4B), which in Drosophila ruptures without disassembling completely (Tates 1971; Stafstrom and Staehelin 1984).

thumbnail

Figure 4. The Place of Noncentrosomal Microtubule Nucleation

The initial stages of noncentrosomal microtubule nucleation revealed by an endogenous GFP–α-tubulin fusion (left) and phase contrast (right). Following the corresponding videos, it is possible to unmistakably tell the chromosomes (arrows) apart form the other phase-dark objects that are present over the nuclear region (asterisks). The cell in (A) is shown as a single timeframe and the cell in (B) as a time-lapse series. In both cells, noncentrosomal microtubule nucleation (arrowheads) takes place close to the remains on the NE and does not overlap with the major chromosomes. Nucleation sites can be clustered (A) or dispersed (B). In the time-lapse series (B), only the chromosomes that are in focus are labelled. Timepoint 0 min in these series corresponds to the first sign of noncentrosomal microtubule nucleation, around 11 min after NEB. A white bar marks the growing end of a microtubule bundle that at timepoint 93 min reaches one of the bivalents.

doi:10.1371/journal.pbio.0020008.g004

The Anastral Spindles Organised in Cells with Membrane-Bound Centrosomes Can Sustain Some Degree of Chromosome Segregation

We then decided to study in more detail the extent to which the anastral spindles organised in cells with membrane-bound centrosomes can mediate successful cell division. To this end, we produced transgenic flies carrying a GFP–α-tubulin fusion together with a His2AvD–YFP (yellow fluorescent protein) strain so that both chromosomes and microtubules could be visualised in the same cell (Figure 5; Video S5). In asp cells, during prometaphase, the bivalents do not move to the extent that they do in control cells (data not shown). As mentioned before, congression occurs (Figure 5; Video S6), but orientation is rarely bipolar. Homologue chromosomes separate at the onset of anaphase, but they barely move, remaining near the center of the spindle. Moreover, they tend to cosegregate (Video S7) and end up included in the same daughter nucleus. All together, these abnormalities result in high levels of aneuploidy in agreement with previous genetic analysis data (Ripoll et al. 1985). In contrast, in half (52%) of the anastral spindles assembled following transient colcemid treatment, homologue chromosomes could be seen to segregate from one another (Figure 5; Video S8). Anaphase in these cells is not complete, however, because only the chromosome-to-pole movement (anaphase A) is observed. The further separation achieved in wild-type cells by the extension of the spindle (anaphase B) is very limited in these cells.

thumbnail

Figure 5. Chromosome Segregation in Anastral Spindles in Drosophila Spermatocytes

(Control [Video S5]) At metaphase I (0), the bivalents (revealed by a His2Avd–YFP fusion, shown by double arrowheads) are aligned in the middle of the spindle (revealed by a GFP–α-tubulin fusion), at the metaphase plate. At the onset of anaphase (3 min), the homologue chromosomes start to migrate towards opposite poles (single arrowheads) and to decondense. During anaphase B (4 min and 6 min), the spindle poles move apart from each other and the two sets of decondensed chromosomes become further separated.

(asp [Video S6]) At timepoint 0, the bivalents align at the metaphase plate. Homologue chromosomes split apart at the onset of anaphase I (4 min). However, anaphase A migration is highly impaired. By the time the chromosomes start to decondense, they have barely moved towards the spindle poles (8 min and 14 min), and often homologue chromosomes end up included in the same daughter nucleus.

(Colcemid [Video S8]) As in asp spermatocytes, the asters (arrows) remain at the plasma membrane at metaphase I in colcemid-treated cells, and the bivalents align in a metaphase plate-like within the acentrosomal spindles (0 min). Homologue chromosomes split apart at the onset of anaphase (upper cell, 6 min) and significantly segregate from one another (upper cell, 8 min; lower cell, 3 min). Further separation of the daughter nuclei during anaphase B is very limited in these cells (8 min), and cytokinesis does not occur.

doi:10.1371/journal.pbio.0020008.g005

The Orientation of the Cytokinesis Furrow Correlates with the Position of the Membrane-Bound Asters, Independently of Spindle Orientation

Following colcemid treatment, we have never observed complete cytokinesis. However, as reported before (Riparbelli et al. 2002), cytokinesis does proceed to completion in around half (47%, n = 19) of asp cells. These cells, which contain unconnected centrosomal asters and anastral spindles, provide a valuable experimental system to assess the contribution of asters and spindle to specifying the place of cleavage. To this end, we plotted the angles between the line defined by the two asters, the major spindle axis, and the plane of cleavage in wild-type and asp spermatocytes (Figure 6). Two conclusions can be drawn from these data. Firstly, the anastral spindles assembled in asp cells can be observed at any angle, even up to 90°, with respect to the position of the two asters. Interestingly, in most such cases, furrow progression forces the spindle to rotate and align with the asters so that, at the end, a fairly normal cytokinesis takes place. Secondly, the orientation of the plane of cleavage keeps a tight 90° ± 10° with respect to the axis defined by the asters and does not correlate with the orientation of the anastral spindle.

thumbnail

Figure 6. Correlation between the Orientation of the Cytokinesis Furrow, the Asters, and the Spindle in asp Spermatocytes

Schematic representation (A–C) of the relative position of the asters (red), the spindle (yellow), and the cytokinesis furrow (blue), corresponding to a control cell (D) and two examples of asp mutant spermatocytes (E and F), respectively. Asters (arrows) and spindles are labelled with a GFP–α-tubulin fusion. The position of the cleavage furrow (double-headed arrow) was determined by time-lapse imaging of these cells (data not shown). In wild-type cells (n = 10), plotting spindle and furrow orientation relative to the interastral axes shows that asters and spindle are tightly aligned, and cleavage occurs at an angle of 90° ± 10° with respect to them (G). In asp spermatocytes (n = 10), the plane of cleavage occurs at a 90° ± 10° angle with respect to the asters and does not correlate with the orientation of the anastral spindle.

doi:10.1371/journal.pbio.0020008.g006

Discussion

We have found that when the asters are kept near the plasma membrane during meiosis I in Drosophila spermatocytes, noncentrosomal microtubules appear over the nuclear region and, in a fraction of the cells, are sorted into anastral bipolar spindles (summarised in Figure 7). Identical observations are derived whether centrosomes are forced to remain membrane-bound by mutation in asp or by transient colcemid treatment of wild-type cells. The very different nature of these two experimental conditions strongly argues against these spindles being assembled as a consequence of the experimental conditions themselves. It rather suggests that the observation of anastral spindles is due to the impaired ability of the plasma membrane-bound centrosomes to contribute to spindle assembly. Anastral spindles are also assembled following the removal of centrosomes by laser ablation or microdissection in cultured cells (Khodjakov et al. 2000; Hinchcliffe et al. 2001) or by inhibiting the formation of centrosomes in mutant Drosophila embryos (Megraw et al. 1999; Vaizel-Ohayon and Schejter 1999), thus reinforcing this argument.

thumbnail

Figure 7. Noncentrosomal Microtubules and Spindle Assembly

(Central column) Spindle assembly in Drosophila spermatocytes with membrane-bound centrosomes. At the time of NEB, the chromatin (pale blue) starts to condense, and the membrane-bound centrosomes (red) organise asters (yellow) at a significant distance from the nuclear region. Around 12 min after NEB, the first noncentrosomal microtubules (green) start to nucleate near the remnants of the NE (grey), as the chromosomes achieve full condensation (dark blue). These microtubules then bundle, associate with the chromosomes, and eventually end up organised into a bipolar anastral array whose shape is reminiscent of the female meiotic spindle.

(Left column) Spindle assembly in wild-type Drosophila oocytes (Theurkauf and Hawley 1992; Matthies et al. 1996). NEB starts at the beginning of stage 13 of oocyte development. At this stage, the oocyte does not contain centrosomes and the chromosomes (karyosome) are tightly condensed (dark blue). Microtubules (green) appear 11–15 min after NEB within the nuclear region in association with the karyosome. These microtubules form bundles and are sorted around the chromatin into a bipolar spindle. Evidence suggests that ER components may be required for spindle assembly in these cells (Kramer and Hawley 2003). At metaphase I, recombined bivalents are aligned at the spindle equator, while those that have not recombined are found closer to the spindle poles. Meiosis remains arrested at this point (stage 14) until oocyte activation. Despite the obvious morphological similitude, the equivalence between these and the anastral spindles organised in spermatocytes with membrane-bound centrosomes is unclear.

(Right column) Hypothesis regarding the contribution of centrosomal and noncentrosomal microtubules to spindle assembly during meiosis I in wild-type Drosophila spermatocytes. Before NEB, the centrosomes are located at opposite positions near the nucleus. Shortly after NEB, astral microtubules enter the nuclear region and make the first contact with the condensing chromatin. No evidence of noncentrosomal microtubule polymerisation near the nuclear region at this stage has been found yet. Once chromosomes are fully condensed, microtubule bundles of centrosomal origin (yellow) connecting centrosomes to chromosomes already exist. At this stage, noncentrosomal microtubules (green) start to polymerise in association with the remnants of the NE. These microtubules form bundles that interact with the chromosomes and intermingle with the microtubules of centrosomal origin. The fully mature spindle in these cells would therefore contain a spindle-shaped structure made of microtubules of noncentrosomal origin (green) embedded in another spindle-shape array made of two overlapping asters (yellow). We propose that each of these subsets may perform to a certain extent some of the functions carried out by normal spindles, but neither of them can on its own mediate robust cell division.

doi:10.1371/journal.pbio.0020008.g007

The Place of Noncentrosomal Microtubule Nucleation

Noncentrosomal microtubule nucleation during cell division is thought to take place over the chromatin (Nachury et al. 2001). This assumption is largely based on the observations carried out in the few acentrosomal systems in which spindle assembly has been followed by time-lapse microscopy (reviewed in Karsenti and Vernos 2001). These include wild-type Drosophila female meiocytes (Theurkauf and Hawley 1992; Matthies et al. 1996), parthenogenetic Sciara embryos (de Saint Phalle and Sullivan 1998), and Xenopus egg extracts (Heald et al. 1996). It is also consistent with the active role of chromosomes in spindle organisation. For instance, it has been reported that bivalents micromanipulated away from the spindle in Drosophila spermatocytes induce the assembly of anastral minispindles in the cytoplasm (Church et al. 1986). Likewise, the removal of chromosomes before NEB has been shown to inhibit spindle assembly in grasshopper spermatocytes (Zhang and Nicklas 1995), although the phenotype of fusolo mutants, recently described, seems to argue otherwise (Bucciarelli et al. 2003). Moreover, the chromosomal localisation of the RanGEF RCC1 is expected to result in a local enrichment of the GTP-bound form of Ran, known to facilitate spindle assembly (Nachury et al. 2001; Wilde et al. 2001; Gruss et al. 2002; reviewed in Hetzer et al. 2002), thus providing a mechanistic interpretation for the suspected role of chromatin in this process.

In contrast, our observations reveal that nucleation of the noncentrosomal microtubules over the nuclear region occurs over the remnants of the NE, which in Drosophila are present throughout cell division despite extensive fenestration at the onset of prometaphase (Tates 1971; Stafstrom and Staehelin 1984; Church and Lin 1985). However, upon closer examination, our observations may also be consistent with the literature quoted above. The single bivalents that organise minispindles when micromanipulated into the cytoplasm in Drosophila spermatocytes have actually been shown to be surrounded by masses of stacked membranes, whose contribution to microtubule nucleation/stabilisation, according to the authors themselves, cannot be ruled out (Church et al. 1986). In Drosophila oocytes, too, the meiotic spindle is ensheathed in a membrane structure derived from the endoplasmic reticulum (ER). Although the bulk of microtubules has been described by time-lapse confocal microscopy to form over the chromatin (Theurkauf and Hawley 1992; Matthies et al. 1996), the contribution of these membranes to the initial stages of microtubule nucleation cannot be discarded either. In this regard, the recent cloning of Axs, which encodes a transmembrane protein associated with the membranes that surround the spindle and is required for the segregation of achiasmate chromosomes, is very tantalising (Kramer and Hawley 2003). Asx is distributed within the ER of the germinal vesicle just before meiotic spindle assembly. Upon germinal vesicle breakdown (GVBD), Axs associates with the developing spindle through all stages of assembly. These observations have been taken as an indication that the ER may be organised into structures that impinge on spindle assembly during meiosis in Drosophila females (Kramer and Hawley 2003), very much in line with our observations in Drosophila spermatocytes.

Indeed, our results do not discard the contribution of chromatin to microtubule stabilisation and sorting into a bipolar array, even if chromatin itself is not the place of initial microtubule nucleation, nor do they rule out the possibility of microtubules being polymerised over the chromatin at later stages. They merely show that in these cells, the remaining bits of the fenestrated NE provide a particularly favourable environment to sustain the initial stages of noncentrosomal microtubule nucleation. Moreover, these observations strongly suggest that, unlike centrosomes, the foci of microtubule nucleation over the nuclear region do not behave as stable MTOCs. As soon as the microtubule bundles acquire a certain length, they interact with the condensed chromosomes and are often sorted into a bipolar spindle, regardless of the initial number of nucleation sites. We have not been able to detect γ-Tub23C at these nucleation sites.

We still do not know the actual contribution of Ran to spindle assembly in Drosophila spermatocytes, although, given its known conservation across distant species (Hetzer et al. 2002), it is likely to play a major role. Since orthologues of most of the known components of this pathway are known in Drosophila, it is technically possible to address this question both under normal conditions and in cells in which centrosomes cannot contribute to spindle assembly as described in this work. Experiments are underway in our laboratory to address these points.

Functional Relevance of the Anastral Spindles

Perhaps the most fundamental question regarding the anastral spindles organised in cells that normally contain centrosomes is the extent to which they could provide a back-up, able to mediate successful and robust cell division when the centrosomes cannot contribute to spindle assembly. Our observations suggest that this is an unlikely scenario. Firstly, only a fraction of cells display a single bipolar array, the rest being accounted for by cases in which either the spindle is multipolar or there is more that one per cell or there is no spindle at all. Secondly, chromosome segregation is also significantly less efficient than in control cells. These two points, however, carry a caveat since they could reflect the effect of depleted asp function or residual traces of active colcemid, rather than the anastral nature of the spindle. Finally, cytokinesis is severely disrupted in these cells. Around half of asp spermatocytes containing anastral spindles go through and complete cytokinesis. However, in these cells, the orientation of the cleavage furrow correlates tightly with the position of the two asters and not at all with the orientation of the spindle. This situation gives rise to cases in which the plane of cleavage is nearly parallel to the spindle. In colcemid-treated cells that contain notoriously small asters, cytokinesis does not occur. These observations strongly argue that asters contribute to specify the place of furrow and may be required for cleavage. The contribution of centrosomes to ensure proper cytokinesis has been previously observed in vertebrate cell lines (Rieder et al. 1997; Savoian et al. 1999; Hinchcliffe et al. 2001; Khodjakov and Rieder 2001), human cell lines (Gromley et al. 2003), Dyctiostelium (Neujahr et al. 1998), or Xenopus (Takayama et al. 2002). This conclusion, however, is not consistent with the observation that cytokinesis is not inhibited in asterless Drosophila spermatocytes (Bonaccorsi et al. 1998).

Therefore, the anastral spindles organised in spermatocytes with membrane-bound centrosomes seem able to provide only some of the functions required for cell division, with relatively low efficiency. The functionality of the anastral spindles assembled in embryos laid by cnn mutant females, which do not appear to contain centrosomes, is also compromised. These spindles are not always properly shaped, the chromosomes are not tightly aligned at the spindle equator, chromosome movements are nonsynchronous, and their segregation not always faithful (Megraw et al. 1999; Vaizel-Ohayon and Schejter 1999). Thus, in this instance, too, when centrosome function is abrogated in a syncytium that normally contains centrosomes and that does not naturally undergo parthenogenesis, anastral spindles can be assembled that are able to perform some of the functions of their wild-type counterparts, but in a rather inefficient manner.

Origin of the Anastral Spindles: Neomorphic or Constitutive

Two alternative interpretations can account for the origin of the anastral spindles that we have observed (Khodjakov et al. 2000). First, they could be neomorphic structures, assembled through a pathway normally repressed that is only triggered in response to the impaired contribution of centrosomal microtubules. Although we cannot at the moment discard this interpretation, we find it hard to envisage how such an alternative pathway could have evolved, given the extremely low frequency of centrosome loss or inactivation in wild-type populations. Moreover, it is also difficult to imagine what sort of signalling mechanism could trigger the alternative pathway in these cells since centrosomes are still present and active as MTOCs.

Alternatively, these anastral microtubule arrays could be a constitutive component of wild-type spindles, normally masked by the abundance of centrosome-derived microtubules, but revealed when asters are kept away. This interpretation is summarised in Figure 7. In wild-type spermatocytes under normal conditions, the first astral microtubules enter the nuclear area shortly after NEB and start to build a bipolar spindle as chromosome condensation progresses. By the time chromosome condensation is fully achieved, a distinct bipolar spindle can be observed in these cells. However, it is not yet fully mature, as the number of microtubules will still increase until anaphase onset. It is about this time that nucleation of the acentrosomal microtubules occurs in cells with plasma membrane-bound centrosomes. Therefore, if this process occurs at the same time in wild-type cells, the acentrosomal microtubules could significantly contribute to the maturation of the cell division spindle. This interpretation is consistent with the recent proposal put forward by Gruss et al. (2002) to account for their observations regarding spindle assembly in HeLa cells. They found that when the function of the human homologue of TPX2 is inhibited by RNA interference, the centrosomal asters do not interact and do not form a spindle. From these observations, they concluded that, intermingled with microtubules of centrosomal origin, the mitotic spindle may contain noncentrosomal microtubules that are stabilised and organised by the chromatin and are essential for the assembly of functional spindles. In Drosophila, secondary spermatocytes' mutation in fusolo seem to reveal the centrosome-derived component of the spindle (Bucciarelli et al. 2003). Forcing the asters away from the nucleus in Drosophila primary spermatocytes reveals the noncentrosomal component that, indeed, does not require asters to get organised into a spindle-like structure. We propose that both components are required to mediate robust cell division. The very recent finding of peripheral, noncentrosomal microtubules that contribute to spindle assembly in LLCPK1α cells provides additional evidence to substantiate this conclusion (Tulu et al. 2003).

Regardless of their neomorphic or constitutive nature, the acentrosomal spindles that we have found in asp and colcemid-treated spermatocytes are, from a morphological point of view, closely reminiscent of the anastral female meiotic spindles found in many animal species, including Drosophila (Theurkauf and Hawley 1992). The same holds true for the anastral spindles assembled when centrosomes are removed from the cell or cannot be organised due to mutation in essential centrosomal components (Megraw et al. 1999; Khodjakov et al. 2000; Khodjakov and Rieder 2001; Hinchcliffe et al. 2001). In the case of the Drosophila female meiotic spindle, the timing of microtubule nucleation is also very similar: between 9 and 12 min after NEB in spermatocytes and 11 to 15 min in oocytes (Matthies et al. 1996). These similarities have led some to propose that experimentally induced anastral spindles could require the same motors and structural components that build the spindles in female meiocytes (Megraw et al. 1999; Khodjakov et al. 2000) (summarised in Figure 6). In fact, it has been suggested that the absence of some of these components at the time syncytial divisions occur could explain the lack of robustness of the anastral spindles assembled in embryos derived from cnn mothers (Megraw et al. 1999). We still do not know to what extent the anastral spindles of spermatocytes share components with the oocyte spindle. Some essential ones cannot be shared, though, since they are only expressed in the female germline. Given the wealth of probes and mutants available in Drosophila, it should be possible to draw a clear picture of the situation regarding this fundamental question.

Materials and Methods

Fly stocks.

Flies from w1118;e11 aspE3/TM6C and w1118;red aspL1/TM6C stocks were crossed to generate w1118;e11 aspE3/red aspL1 transheterozygous individuals. The viability of aspE3/aspL1 males is high, but they are poorly fertile and produce high levels of aneuploid gametes.

Transgenes.

The chromosomes were labeled with transgenes expressing either a His2avD–GFP fusion (Clarkson and Saint 1999) or its derivative, His2avD–EYFP, constructed by us under the control of the polyubiquitin promoter (Lee et al. 1988). To visualise centrioles, we used the transgene expressing GFP-PACT (pericentrin-AKAP450 centrosomal targeting) (kindly provided by J. Raff) that contains the predicted Drosophila homologue of the PACT domain described by Gillingham and Munro (2000). To visualise microtubules, we constructed a transgene that contained the GFP–α-tub84B fusion as previously described (Grieder et al. 2000) under the control of the polyubiquitin promoter (Lee et al. 1988).

Time-lapse recording.

Live spermatocytes were recorded as previously described (Rebollo and Gonzalez 2000, 2003). For most applications, we collected a series of timepoints at 15–30 s intervals, each containing four to eight XY sections at different depths along the Z axis and including both the phase-contrast and fluorescence channels. For more-detailed 3D reconstruction, stacks containing 20 sections were obtained. Laser intensity was always kept to a minimum, and only the excitation laser line 488 was utilised. GFP and YFP signals were distinguished by overlying the two recorded channels. Image processing was performed with NIH-Scion Image, Interactive Data Language (IDL), and huygens2. For 3D reconstructions, we wrote macros in NIH-Scion Image to navigate through the three dimensions of the cell stack.

Colcemid treatment.

Newly hatched adult males were fed for 8–12 h with a solution containing 32 μg/ml of colcemid (Sigma, St. Louis, Missouri, United States) in 1 M sucrose. Upon dissection, their testes were prepared for in vivo imaging as described above. Once under the microscope, microtubules were allowed to repolymerise by a 1-s pulse of 350 nm light that inactivates the drug. For simplicity, this entire procedure of exposure to colcemid followed by light inactivation of the drug is referred to through this manuscript as ‘colcemid treatment.’

Supporting Information

Accession Numbers

The FlyBase accession numbers discussed in this paper are α-tubulin 84B (CG1913), asp (CG6875), Axs (CG9703), cnn (CG4832), γ-Tub23C (CG3157), His2AvD (CG5499). The GeneBank accession numbers discussed in this paper areGFP (U57609.1), Ran (NM_006325), RCC1 (D00679), TPX2 (BC020207), and YFP (U57609.1).

Video S1. Centriole Migration in Primary Spermatocytes

doi:10.1371/journal.pbio.0020008.s001

(849 KB MOV)

Video S2. Spindle Assembly in Control Spermatocytes

doi:10.1371/journal.pbio.0020008.s002

(442 KB MOV)

Video S3. Spindle Assembly in asp Spermatocytes

doi:10.1371/journal.pbio.0020008.s003

(675 KB MOV)

Video S4. Spindle Assembly in Colcemid-Treated Spermatocytes

doi:10.1371/journal.pbio.0020008.s004

(844 KB MOV)

Video S5. Chromosome Segregation in Control Spermatocytes

doi:10.1371/journal.pbio.0020008.s005

(206 KB MOV)

Video S6. Chromosome Segregation in asp Spermatocytes I

doi:10.1371/journal.pbio.0020008.s006

(452 KB MOV)

Video S7. Chromosome Segregation in asp Spermatocytes II

doi:10.1371/journal.pbio.0020008.s007

(377 KB MOV)

Video S8. Chromosome Segregation in Colcemid-Treated Spermatocytes

doi:10.1371/journal.pbio.0020008.s008

(465 KB MOV)

Acknowledgments

We are grateful to W. Theurkauf for advice on the method to inactivate colcemid; to J. W. Raff for providing the Drosophila GFP-PACT construct; to T. Zimmerman for his help with image processing; to A. M. Voie for performing embryo injections; and to I. Vernos and E. Karsenti for very helpful comments on this manuscript. Our lab is financed by grants from the European Union and the Ramon Areces Foundation.

Author Contributions

ER and CG conceived and designed the experiments. ER performed the experiments. ER and CG analyzed the data. ER, SL, and JR contributed reagents/materials/analysis tools. ER and CG wrote the paper.

References

  1. 1. Bonaccorsi S, Giansanti MG, Gatti M (1998) Spindle self-organization and cytokinesis during male meiosis in asterless mutants of Drosophila melanogaster. J Cell Biol 142: 751–761.
  2. 2. Bonaccorsi S, Giansanti MG, Gatti M (2000) Spindle assembly in Drosophila neuroblasts and ganglion mother cells. Nat Cell Biol 2: 54–56.
  3. 3. Bornens M (2002) Centrosome composition and microtubule anchoring mechanisms. Curr Opin Cell Biol 14: 25–34.
  4. 4. Bucciarelli E, Giansanti MG, Bonaccorsi S, Gatti M (2003) Spindle assembly and cytokinesis in the absence of chromosomes during Drosophila male meiosis. J Cell Biol 160: 993–999.
  5. 5. Casal J, Gonzalez C, Wandosell F, Avila J, Ripoll P (1990) Abnormal meiotic spindles cause a cascade of defects during spermatogenesis in asp males of Drosophila. Development 108: 251–260.
  6. 6. Cenci G, Bonaccorsi S, Pisano C, Verni F, Gatti M (1994) Chromatin and microtubule organization during premeiotic, meiotic and early postmeiotic stages of Drosophila melanogaster spermatogenesis. J Cell Sci 107(Pt 12): 3521–3534.
  7. 7. Church K, Lin HP (1982) Meiosis in Drosophila melanogaster. II. The prometaphase-I kinetochore microtubule bundle and kinetochore orientation in males. J Cell Biol 93: 365–373.
  8. 8. Church K, Lin HP (1985) Kinetochore microtubules and chromosome movement during prometaphase in Drosophila melanogaster spermatocytes studied in life and with the electron microscope. Chromosoma 92: 273–282.
  9. 9. Church K, Nicklas RB, Lin HP (1986) Micromanipulated bivalents can trigger mini-spindle formation in Drosophila melanogaster spermatocyte cytoplasm. J Cell Biol 103: 2765–2773.
  10. 10. Clarkson M, Saint RA (1999) His2AvDGFP fusion gene complements a lethal His2AvD mutant allele and provides an in vivo marker for Drosophila chromosome behavior. DNA Cell Biol 18: 457–462.
  11. 11. Compton DA (2000) Spindle assembly in animal cells. Annu Rev Biochem 69: 95–114.
  12. 12. de Saint Phalle B, Sullivan W (1998) Spindle assembly and mitosis without centrosomes in parthenogenetic Sciara embryos. J Cell Biol 141: 1383–1391.
  13. 13. do Carmo Avides M, Glover DM (1999) Abnormal spindle protein, Asp, and the integrity of mitotic centrosomal microtubule organizing centers. Science 283: 1733–1735.
  14. 14. Gillingham AK, Munro S (2000) The PACT domain, a conserved centrosomal targeting motif in the coiled-coil proteins AKAP450 and pericentrin. EMBO Rep 1: 524–529.
  15. 15. Gonzalez C, Saunders RD, Casal J, Molina I, Carmena M, et al. (1990) Mutations at the asp locus of Drosophila lead to multiple free centrosomes in syncytial embryos, but restrict centrosome duplication in larval neuroblasts. J Cell Sci 96(Pt 4): 605–616.
  16. 16. Grieder NC, de Cuevas M, Spradling AC (2000) The fusome organizes the microtubule network during oocyte differentiation in Drosophila. Development 127: 4253–4264.
  17. 17. Gromley A, Jurczyk A, Sillibourne J, Halilovic E, Mogensen M, et al. (2003) A novel human protein of the maternal centriole is required for the final stages of cytokinesis and entry into S phase. J Cell Biol 161: 535–545.
  18. 18. Gruss OJ, Wittmann M, Yokoyama H, Pepperkok R, Kufer T, et al. (2002) Chromosome-induced microtubule assembly mediated by TPX2 is required for spindle formation in HeLa cells. Nat Cell Biol 4: 871–879.
  19. 19. Heald R, Tournebize R, Blank T, Sandaltzopoulos R, Becker P, et al. (1996) Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts. Nature 382: 420–425.
  20. 20. Hetzer M, Gruss OJ, Mattaj IW (2002) The Ran GTPase as a marker of chromosome position in spindle formation and nuclear envelope assembly. Nat Cell Biol 4: E177–E184.
  21. 21. Hinchcliffe EH, Miller FJ, Cham M, Khodjakov A, Sluder G (2001) Requirement of a centrosomal activity for cell cycle progression through G1 into S phase. Science 291: 1547–1550.
  22. 22. Karsenti E, Vernos I (2001) The mitotic spindle: A self-made machine. Science 294: 543–547.
  23. 23. Khodjakov A, Rieder CL (2001) Centrosomes enhance the fidelity of cytokinesis in vertebrates and are required for cell cycle progression. J Cell Biol 153: 237–242.
  24. 24. Khodjakov A, Cole RW, Oakley BR, Rieder CL (2000) Centrosome-independent mitotic spindle formation in vertebrates. Curr Biol 10: 59–67.
  25. 25. Kramer J, Hawley RS (2003) The spindle-associated transmembrane protein Axs identifies a membranous structure ensheathing the meiotic spindle. Nat Cell Biol 5: 261–263.
  26. 26. Lee HS, Simon JA, Lis JT (1988) Structure and expression of ubiquitin genes of Drosophila melanogaster. Mol Cell Biol 8: 4727–4735.
  27. 27. Matthies HJ, McDonald HB, Goldstein LS, Theurkauf WE (1996) Anastral meiotic spindle morphogenesis: Role of the nonclaret disjunctional kinesin-like protein. J Cell Biol 134: 455–464.
  28. 28. McKim KS, Hawley RS (1995) Chromosomal control of meiotic cell division. Science 270: 1595–1601.
  29. 29. Megraw TL, Li K, Kao LR, Kaufman TC (1999) The centrosomin protein is required for centrosome assembly and function during cleavage in Drosophila. Development 126: 2829–2839.
  30. 30. Megraw TL, Kao LR, Kaufman TC (2001) Zygotic development without functional mitotic centrosomes. Curr Biol 11: 116–120.
  31. 31. Nachury MV, Maresca TJ, Salmon WC, Waterman-Storer CM, Heald R, et al. (2001) Importin beta is a mitotic target of the small GTPase Ran in spindle assembly. Cell 104: 95–106.
  32. 32. Neujahr R, Albrecht R, Kohler J, Matzner M, Schwartz JM, et al. (1998) Microtubule-mediated centrosome motility and the positioning of cleavage furrows in multinucleate myosin II-null cells. J Cell Sci 111(Pt 9): 1227–1240.
  33. 33. Raff JW (2001) Centrosomes: Central no more? Curr Biol 11: R159–R161.
  34. 34. Rebollo E, Gonzalez C (2000) Visualizing the spindle checkpoint in Drosophila spermatocytes. EMBO Rep 1: 65–70.
  35. 35. Rebollo E, Gonzalez C (2004) Time-lapse imaging of Drosophila male meiosis by phase-contrast and fluorescence microscopy. Methods Mol Biol 247: 77–87.
  36. 36. Rieder CL, Khodjakov A, Paliulis LV, Fortier TM, Cole RW, et al. (1997) Mitosis in vertebrate somatic cells with two spindles: Implications for the metaphase/anaphase transition checkpoint and cleavage. Proc Natl Acad Sci U S A 94: 5107–5112.
  37. 37. Riparbelli MG, Callaini G, Glover DM, Avides Mdo C (2002) A requirement for the abnormal spindle protein to organise microtubules of the central spindle for cytokinesis in Drosophila. J Cell Sci 115: 913–922.
  38. 38. Ripoll P, Pimpinelli S, Valdivia MM, Avila J (1985) A cell division mutant of Drosophila with a functionally abnormal spindle. Cell 41: 907–912.
  39. 39. Saunders RD, Avides MC, Howard T, Gonzalez C, Glover DM (1997) The Drosophila gene abnormal spindle encodes a novel microtubule-associated protein that associates with the polar regions of the mitotic spindle. J Cell Biol 137: 881–890.
  40. 40. Savoian MS, Earnshaw WC, Khodjakov A, Rieder CL (1999) Cleavage furrows formed between centrosomes lacking an intervening spindle and chromosomes contain microtubule bundles, INCENP, and CHO1 but not CENP-E. Mol Biol Cell 10: 297–311.
  41. 41. Savoian MS, Goldberg ML, Rieder CL (2000) The rate of poleward chromosome motion is attenuated in Drosophila zw10 and rod mutants. Nat Cell Biol 2: 948–952.
  42. 42. Stafstrom JP, Staehelin LA (1984) Dynamics of the nuclear envelope and of nuclear pore complexes during mitosis in the Drosophila embryo. Eur J Cell Biol 34: 179–189.
  43. 43. Sunkel CE, Gomes R, Sampaio P, Perdigao J, Gonzalez C (1995) Gamma-tubulin is required for the structure and function of the microtubule organizing centre in Drosophila neuroblasts. EMBO J 14: 28–36.
  44. 44. Takayama M, Noguchi T, Yamashiro S, Mabuchi I (2002) Microtuble organization in Xenopus eggs during the first cleavage and its role in cytokinesis. Cell Struct Funct 27: 163–171.
  45. 45. Tates AD (1971) Cytodifferentiation during spermatogenesis in Drosophila melanogaster: An electron microscope study [dissertation]. Rijksuniversiteit de Leiden. Leiden, Netherlands.
  46. 46. Theurkauf WE, Hawley RS (1992) Meiotic spindle assembly in Drosophila females: Behavior of nonexchange chromosomes and the effects of mutations in the nod kinesin-like protein. J Cell Biol 116: 1167–1180.
  47. 47. Tulu US, Rusan NM, Wadsworth P (2003) Peripheral, non-centrosome-associated microtubules contribute to spindle formation in centrosome-containing cells. Curr Biol 13: 1894–1899.
  48. 48. Vaizel-Ohayon D, Schejter ED (1999) Mutations in centrosomin reveal requirements for centrosomal function during early Drosophila embryogenesis. Curr Biol 9: 889–898.
  49. 49. Wakefield JG, Bonaccorsi S, Gatti M (2001) The Drosophila protein Asp is involved in microtubule organization during spindle formation and cytokinesis. J Cell Biol 153: 637–648.
  50. 50. Wilde A, Lizarraga SB, Zhang L, Wiese C, Gliksman NR, et al. (2001) Ran stimulates spindle assembly by altering microtubule dynamics and the balance of motor activities. Nat Cell Biol 3: 221–227.
  51. 51. Zhang D, Nicklas RB (1995) Chromosomes initiate spindle assembly upon experimental dissolution of the nuclear envelope in grasshopper spermatocytes. J Cell Biol 131: 1125–1131.
  52. 52. Zheng Y, Jung MK, Oakley BR (1991) Gamma-tubulin is present in Drosophila melanogaster and Homo sapiens and is associated with the centrosome. Cell 65: 817–823.