Figures
Since publication of this paper, the authors became aware of several incorrect or missing details that required correcting. The authors have provided corrected versions, along with explanations, here.
- Figure 1A-D have been used again in Figures S2B, S2F and S2H. This was deliberate, and on request of a referee for more detail, but we did not explicitly mention it in the figure legend. The corrected figure legend for Figure S2 has been provided here.
- Figure 2E: an RT-PCR experiment is shown in which overexpression of an artificial microRNA against RBR (amigo-RBR) reduces RBR RNA levels. We used two different promoters for overexpression, pG1090 XVE and 35S, and both yielded exactly the same result. We inadvertently used results for pG1090 XVE and named them 35S::amiGO-RBR in the original figure, and have now replaced these with the results for 35S::amiGO-RBR. In addition, because samples were run in separate slots on the same gel, we put a white horizontal line between the RBR and ACTIN bands. The corrected Figure 2 file has been provided here.
- Figure 3M uses an image that was shown again in Figure S1J for different features. We now indicate this explicitly in corrected Figure 3 and Figure S1 legends, which are shown here.
- Figure 3J and Figure 3T inadvertently contained images that we had previously published elsewhere, focusing on different aspects of the phenotype that emerged from the analysis of the same set of data. In the corrected Figure 3, provided here, we have replaced these panels with other representative images from the same data set which are not used elsewhere. The replicate data and choice of appropriate alternate panels for this correction was overseen by our institution.
The AmiGO strategy. TS, Target sequence (A). Root apical meristem (RAM) of WT (B), 35S:amiGORBR at 8 (C) and 12 (D) dpg seedlings. Validation of the amiGORBR RNA interference by RT-PCR detection of endogenous RBR transcripts (E) and Western blot analyses for RBR protein levels (F). Mature amiGO-RBR synthesis detected by small-RNA Northern blot (G). The in planta action of amiGO-RBR was revealed by introducing the sensor construct 35S::vYFP:amiGORBR-TS in WT (H) and in the pRCH1::amiGORBR (J) backgrounds. Expression pattern of the pRCH1::GFP marker in the WT background (I) [54]. AmiGORBR expression driven by different promoters generates distinct phenotypes in primary root meristems of 12 dpg seedlings. (K) p35S::amiGORBR causes overproliferation of QC, LRC (arrow heads), and CSC (asterisks) as well as cell death in vascular and columella cells. (L) pRCH1::amiGORBR shows overproliferation of the QC and LRC and cell death and (M) pSCR::amiGORBR shows extra periclinal divisions of the ground tissue (arrow heads) and extra division of the QC (asterisks), while (N) pWOX5::amiGORBR;pWOX5::GFP shows QC divisions that cause an increase in columella layers (asterisks). See also Figures S3, S4, S5.
Expression patterns of cell-fate markers in stem cell niche of WT (A, C, and E) and pWOX5:amiGORBR (B, D, and F) 5 dpg seedlings. Marker genotypes: cytoplasmic pWOX5:GFP and nuclear pSCR:SCR:YFP (A and B), pSMB::SMB:GFP (C and D), cytoplasmic pWOX5:GFP; nuclear pSCR:SCR:YFP and membrane pACR4:ACR4:GFP (E and F). Arrows in (D) indicate shift in SMB expression, and arrows in (E) and (F) point to plasma membrane-localized ACR4-GFP expression. Full rescue of the QC division in pWOX5:amiGORBR complemented with pWOX5:RBR:YFP and scr-4 complemented with pSCR::SCR:YFP (G, I) and no rescue by LxCxE mutant pWOX5:RBRN849F-YFP or pSCR::SCRAxCxA:YFP (H, J). Root phenotype of 12 dpg seedlings of Col-0 WT (K), pWOX5::amiGORBR (L), pWOX5::amiGORBR;pWOX5::RBR:vYFP (M), pWOX5::amiGORBR;pRBR::RBRN849A:vYFP (N), and pSCR::SCRAxCxA:YFP, scr-4 (O). Asterisks depict the columellla layers rootwards from the layer in contact with the QC and excluding the detaching distal layers. Production of extra columella stem cell in pSCR::SCRAxCxA:YFP, scr-4 as shown with ACR4-GFP marker (P). Number of columella stem cell layers by lugol staining in pSCR::SCRAxCxA:YFP, scr-4 (Q), Col-0 WT (R), scr-4 (S), and pSCR::SCRAxCxA:YFP shr-2 scr-4 plants (T). See also Figure S6 and Figure S1, where Figure 3M is redisplayed for QC identification.
None of these changes affect in any way the results and conclusions reported in the paper.
Supporting Information
Figure S1.
Root meristem in Arabidopsis thaliana. Different cell types in the root apical meristem of Arabidopsis thaliana. Quiescent center, QC; Columella Stem Cell, CSC; Columella differentiated, Col; Lateral Root Cap, LRC; Epidermis, Epi; Cortex, Cor; Endodermis, En; Vasculature, Vasc. Cortex and Endodermis comprise the ground tissue (green); the columella tissue is represented in orange, and QC cells are yellow. (A) SCR expression domain, in QC, ground tissue stem cells and endodermis (B), and WOX5 expression domain in QC of the complemented pWOX5::RBR:YFP pWOX5::amiGORBR line, shown in Fig 3M (C).
https://doi.org/10.1371/journal.pbio.1001997.s001
(TIF)
Figure S2.
QC incorporates F-ara-EdU at longer times than surrounding stem cells. Left images show red (F-ara-EdU) and blue (DAPI staining) channels; right pictures show overlayed green (pSCR::SCR:GFP) channel using images shown in Figure 1 A-D. Arrowhead shows QC region that is stained by pSCR::SCR:GFP. Note that all green nuclei have no F-ara-EdU signal at 1–3 dat, but they show signal at 4 dat. Root meristem shown (A–B) 1 dat, (C–D) 2 dat, (E–F) 3 dat, and (G–H) 4 dat.
https://doi.org/10.1371/journal.pbio.1001997.s002
(TIF)
Reference
Citation: The PLOS Biology Staff (2014) Correction: A SCARECROW-RETINOBLASTOMA Protein Network Controls Protective Quiescence in the Arabidopsis Root Stem Cell Organizer. PLoS Biol 12(10): e1001997. https://doi.org/10.1371/journal.pbio.1001997
Published: October 20, 2014
Copyright: © 2014 The PLOS Biology Staff. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.