Multiple epigenetic marks have been proposed to contribute to the regulation of antigen receptor gene assembly via V(D)J recombination. Here we provide a comprehensive view of DNA methylation at the immunoglobulin heavy chain (IgH) gene locus prior to and during V(D)J recombination. DNA methylation did not correlate with the histone modification state on unrearranged alleles, indicating that these epigenetic marks were regulated independently. Instead, pockets of tissue-specific demethylation were restricted to DNase I hypersensitive sites within this locus. Though unrearranged diversity (DH) and joining (JH) gene segments were methylated, DJH junctions created after the first recombination step were largely demethylated in pro-, pre-, and mature B cells. Junctional demethylation was highly localized, B-lineage-specific, and required an intact tissue-specific enhancer, Eμ. We propose that demethylation occurs after the first recombination step and may mark the junction for secondary recombination.
DNA methylation at CpG dinucleotides is implicated in the regulation of gene expression in mammals. However, the regulation of DNA methylation itself is less clear despite recent advances in identifying enzymes that add or remove methyl groups. We have investigated the dynamics of DNA methylation during genome rearrangements that assemble antigen receptor genes in developing B lymphocytes to determine whether methylation status correlates with rearrangement potential. Two recombination events generate immunoglobulin heavy chain (IgH) genes. The first step brings together diversity (DH) and joining (JH) gene segments to produce DJH junctions. We show that both gene segments are methylated prior to rearrangement, whereas the DJH product is demethylated. DJH junctional demethylation is tissue-specific and requires an enhancer, Eμ, located within the IgH locus. The latter observations indicate that localized demethylation of the DJH junction occurs after the first recombination step and thus does not guide this first step of IgH gene assembly. Our working hypothesis is that recombination induces demethylation of recombinant product and may mark the junction for the second step of IgH rearrangement, juxtaposition of variable (VH) gene segments to rearranged DJH products to produce fully recombined V(D)J alleles.
Citation: Selimyan R, Gerstein RM, Ivanova I, Precht P, Subrahmanyam R, et al. (2013) Localized DNA Demethylation at Recombination Intermediates during Immunoglobulin Heavy Chain Gene Assembly. PLoS Biol 11(1): e1001475. doi:10.1371/journal.pbio.1001475
Academic Editor: David Nemazee, Scripps Research Institute, United States of America
Received: August 10, 2012; Accepted: December 14, 2012; Published: January 29, 2013
This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
Funding: This work was supported by NIH grant AI 2047 (to F.W.A.) and AI 43534 (to R.M.G.), and by the Intramural Research Program of the National Institute on Aging (Baltimore, MD). The U-Mass Medical School Flow Cytometry Core is supported by NIH NIDDK Diabetes Endocrinology Research Center grant 5 P30DK32520. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Abbreviations: CGI, CpG islands; DH, IgH diversity region; DHS, DNase l hypersensitive sites; DP, double positive; H3K4me3, histone H3 trimethylated at lysine 4; H3K9me, histone H3 methylated at lysine 9; H3K9me2, H3 dimethylated at lysine 9; IgH, immunoglobulin heavy chain; JH, IgH joining region; VH, IgH variable region
Tissue-specific gene expression requires multiple epigenetic changes. These include nuclear location, chromatin remodeling, covalent histone modifications, and DNA methylation –. Recent genome-wide analyses reveal several important correlations between gene activity and epigenetic modifications. Transcriptionally inactive genes are marked by histone H3 methylation at lysines 9 and 27 (H3K9me, H3K27me), whereas histone acetylation and H3K4 methylation are associated with gene activity . H3K9 acetylation and H3K4 methylation have been inversely correlated with CpG methylation, consistent with the long-established view that transcriptionally active promoters are hypomethylated .
Most studies of CpG methylation vis-à-vis transcription have focused on gene promoters, particularly those that contain CpG islands (CGI). Such promoters are typically un-methylated and are transcriptionally active; conversely, methylated CGI promoters are usually transcriptionally silent. The role of CpG methylation in non-CGI promoters is less clear . Many tissue-specific promoters fall in this category and, in a limited number of cases where examined, such promoters also have reduced CpG methylation in tissues where they are active. Most recently, the advent of whole genome CpG mapping has drawn attention to possible functions of CpG methylation within gene bodies ,. Proposed functions for such CpGs include transcription elongation and the regulation of splicing. The lack of a coherent picture for the role of CpG methylation in non-CGI contexts indicates an ongoing need for analysis of CpG methylation, particularly in tissue-specific genes that lack CGIs.
Antigen receptor genes of B and T lymphocytes serve as excellent paradigms for developmentally regulated gene expression. Immunoglobulin heavy chain (IgH) genes are assembled during B lymphocyte development by juxtaposition of variable (VH), diversity (DH), and joining (JH) gene segments by a process known as V(D)J recombination . The mouse genome contains approximately 150 VH gene segments, 10 to 13 DH gene segments, and four JH gene segments, dispersed over 2 Mb ,. DH to JH recombination occurs first, followed by VH recombination to the rearranged DJH junction (Figure 1). These two steps produce a fully recombined V(D)J allele, with the potential to encode heavy chain protein. IgH expressing cells develop into pre-B cells where immunoglobulin κ or λ light chain genes rearrange. Cells that make both IgH and IgL polypeptides express cell surface immunoglobulin, and are exported out of the bone marrow to become mature functional B cells.
Figure 1. Immunoglobulin heavy chain locus and B cell development.
Schematic of the murine IgH locus showing variable (VH, blue, n represents approximately 150 VH gene segments), diversity (DH, grey, n represents six to nine DSP gene segments), and joining (JH, orange) gene segment ,. Exons encoding the constant regions of IgM and IgD are indicated as Cμ and Cδ. A promoter 5′ of DQ52, the 3′-most DH gene segment, is indicated by the yellow oval and the intronic enhancer Eμ by a teal oval. Top line shows the germline (GL) configuration with associated histone modifications in B lineage precursors –. Histone H3 and H4 acetylation are shown in orange and presence of heterochromatic H3K9 methylation by the red line. Vertical red arrows represent the tissue-specific DNase I hypersensitive sites in the germline state. Next two lines show sequential stages of VDJ recombination at the IgH locus. DH to JH rearrangement occurs first resulting in a DJH junction and, depending on which DH rearranges, residual upstream unrearranged DHs may be present. VH rearrangement occurs to the DJH junction to generate a VDJH junction; during this process unrearranged DHs are lost from the genome.doi:10.1371/journal.pbio.1001475.g001
DNA methylation was the earliest epigenetic mark associated with immunoglobulin gene regulation ,. Using a variety of cell lines, these pioneering studies showed that Vκ promoters that were rearranged, and therefore transcriptionally active, lacked CpG methylation. Because these studies used restriction enzyme isoschisomers, it was difficult to define the extent of demethylation on recombined κ alleles in these studies; however, a site located approximately 10 kb 5′ of the promoter of a rearranged Vκ21 remained methylated. Subsequently, studies by Bergman, Cedar and colleagues showed that one κ allele is preferentially demethylated at a site close to the Cκ exons at the pre-B cell stage of B cell differentiation. Preferential Vκ recombination of the demethylated allele led them to propose that demethylation permits Vκ to Jκ recombination . Analyses of transgenic mice containing synthetic recombination substitutes were also consistent with the idea that demethylated alleles recombine preferentially ,. Additionally, de-methylation state of this site has also been proposed to play a role in somatic hypermutation in germinal centers .
Compared to these extensive studies at the κ locus, relatively little is known about developmental regulation of DNA methylation at the IgH locus. Two relatively recent studies evaluated the role of methylation in VH gene rearrangements ,. Within the small family of VHS107 genes, the methylation status of one CpG in pro-B cells correlated with recombination potential. Both studies also examined the status of the 3′-most VH gene segment, VH 81X, that rearranges most frequently. Two CpG sites within the coding region remained methylated in pro-B cells whereas a CpG near the recombination signal sequence was demethylated. However, the extent of demethylation of all genes examined was comparable in pro-B cells where VH genes recombine and in non-B lineage cells where VH genes do not recombine. Thus, methylation status could not be directly correlated with rearrangement potential. More importantly, virtually nothing is known about CpG methylation in DH–Cμ part of the locus, where V(D)J recombination is initiated .
Here we used the bisulfite modification method to assay DNA methylation in the DH–Cμ domain of the IgH locus prior to the onset of recombination and as recombination proceeds. The only sites of extensive tissue-specific DNA demethylation in the unrearranged locus corresponded to a promoter close to DQ52 and the intronic enhancer Eμ. Additionally, sequences close to the intergenic control region , which marks the 5′ boundary of the DH-Cμ domain, were partially demethylated. These regions correspond to the only known DNase1 hypersensitive sites in this part of the IgH locus. All CpGs close to, or within, other re-arrangeable DH or JH gene segments remained hypermethylated regardless of whether they were marked with activation or inactivation histone modifications. However, DJH junctions that were generated after the first recombination step were completely demethylated. Junctional demethylation was highly localized, B lineage-specific, and required the presence of an intact Eμ. These observations suggest that localized demethylation of the DJH junction occurs after the first recombination step and may mark the junction for subsequent VH gene rearrangements.
Tissue-Specific DNA Demethylation Coincides with DNase I Hypersensitive Sites
Histone modifications across the unrearranged DH–Cμ domain of the IgH locus show marked heterogeneity. The region encompassing the JH gene segments is marked by the highest levels of histone acetylation and histone H3 trimethylation at lysine 4 (H3K4me3) (Figure 1) –. The 5′- and 3′ DH gene segments, DFL16.1 and DQ52, are also associated with these active modifications, whereas the six to nine DSP gene segments that lie in between are marked by repressive H3 dimethylation at lysine 9 (H3K9me2). Two tissue-specific DNase l hypersensitive sites (DHS) in this part of the locus correspond to a promoter associated with DQ52 and the intronic enhancer Eμ. A cluster of three DHSs was recently identified just 5′ of DFL16.1 . These latter regions contain binding sites for the transcription factor CTCF  and have been proposed to serve as the 5′ boundary of the DH–Cμ domain .
To investigate the DNA methylation state of the unrearranged locus, we purified genomic DNA from pro-B cells obtained from the bone marrow of RAG2-deficient mice to use in bisulfite modification assays modified from Frommer et al. . We used at least two independent DNA preparations starting with cells obtained from six mice for each sample. Methylation profiles of the regions amplified from two independent experiments were comparable (Figure S1). We sequenced approximately 12–50 alleles (Table S1), of which 12 representative clones are shown throughout the text. The mb-1 promoter and β-globin locus control region served as positive  and negative controls , respectively (Figure S2); we used CD4+ CD8+ double positive thymocytes (DP) as a lineage control and kidney genomic DNA as a non-lymphoid control. The relative positions of all CpGs are shown in Figure S3.
We found that sequences near DQ52 and Eμ were substantially demethylated in RAG2-deficient pro-B cell DNA compared to kidney DNA (Figure 2). In contrast, unrearranged DFL16.1 and DSP gene segments, which are marked by active and inactive histone modifications , respectively, were methylated. While the methylation status of DSP gene segments was consistent with earlier association of H3K9me2 modification with CpG methylation , the methylated state of DFL16.1 did not follow this paradigm. An amplicon that included the JH1 gene segment located within the peak of active modifications was partially demethylated in pro-B cells compared to kidney. Note that the DQ52 and JH1 amplicons, that are demethylated and partially methylated, respectively, are separated by only about 170 nucleotides that contain two CpGs. These observations indicate that the prominent peak of H3ac and H3K4me3 encompassing the JH gene segments is not sufficient to direct CpG demethylation of this region. Moreover, the JH region has been shown to bind the highest levels of recombinase proteins RAG1 and 2, to form a recombination center at which IgH rearrangement initiates . Clearly, the partially methylated state of this region does not preclude RAG 1/2 recruitment. Additionally, DQ52 and DFL16.1 have been shown to recombine most frequently among DH gene segments –, yet they had distinctly different methylation states. The methylation status of DH and JH gene segments suggests that the first step of Ig gene assembly may not be regulated by DNA methylation.
Figure 2. DNA methylation in the DH-Cμ domain of the IgH locus.
Purified genomic DNA from CD19+ pro-B cells obtained from the bone marrow of RAG2-deficient mice was used in bisulfite modification assays. 400–500-bp amplicons corresponding to DH and JH gene segments and regulatory sequences (DQ52 and Eμ) as indicated were cloned and sequenced. Each horizontal line represents the methylation status of a sequenced allele and each vertical line methylation of a specific cytosine residue. JH1-associated CpGs are indicated as squares. Filled and open circles, or squares, indicate methylated and unmethylated cytosines, respectively. Methylation at each position is summarized in the form of pie charts with the percentage of methylated residues indicated numerically. Asterisks replace pie charts where the number of sequences falls below 12. Data shown are derived from at least two independent preparations of pooled bone marrow cells obtained from four to six mice each. Kidney genomic DNA served as the non-B lineage control and the previously characterized mb-1 promoter served as a B lineage-specific positive control. Primers for the DSP region amplify all DSP gene segments from the repeat array. Because the numbers of CpGs vary between DSP gene segments, three different CpG patterns are evident in this amplicon.doi:10.1371/journal.pbio.1001475.g002
Because both DQ52 and Eμ coincide with tissue-specific DHSs, we examined the methylation status of newly identified DHSs located approximately 6, 4, and 1 kb 5′ of DFL16.1. We found two clusters of CpG dinucleotides centered approximately at 6.5 and 4 kb 5′ of DFL16.1. The precise location of these regions and the relative location of CpG dinucleotides within each region are shown in Figure S3. The cluster at −6.5 was partially demethylated in primary pro-B cells compared to DP thymocytes that served as a non-B lineage control (Figure 3). The cluster at −4 kb was similarly methylated in both cell types. Because neither location corresponded precisely with the DHSs, we also examined shorter stretches of CpG dinucleotides in this region. Two sites located at −6 kb were significantly demethylated in pro-B cells compared to DP thymocytes, whereas one out of three CpGs at −5 kb was completely demethylated in pro-B cells. Two CpGs at −3 kb were completely demethylated in both pro-B cells and DP thymocytes. Finally, a cluster of CpGs in the −1.3 kb region was largely methylated in pro-B cells (see below). Overall, the majority of sites in this region remained methylated in pro-B cells, with very specific sites being targeted for demethylation. Possible interpretations of these observations are discussed below.
Figure 3. DNA methylation status of DNase 1 hypersensitive sites 5′ of DFL16.1.
Three newly identified DNase 1 hypersensitive sites are located at approximately 6–6.5, 4–4.5, and 0.4–1.3 kb 5′ of DFL16.1. Genomic DNA from primary RAG-deficient pro-B cells and DP (CD4+ CD8+) thymocytes were used in bisulfite mapping experiments to examine CpG methylation. Five amplicons covering regions between 3 kb and 7 kb 5′ of DFL16.1 were modified, cloned, and sequenced. The distribution of CpG dinucleotides within each amplicon are noted in Figure S3. Filled and open circles represent methylated and unmethylated residues, respectively. Pie charts summarize the percentage of methylated alleles at each position.doi:10.1371/journal.pbio.1001475.g003
Localized DNA Methylation at Recombination Intermediates
To determine whether DNA methylation was altered by recombination, we examined pro-B cells purified from the bone marrow of wild-type mice, which contain DJH junctions as well as unrearranged alleles. We found that unrearranged DFL16.1, DSP, and JH regions were methylated, while Eμ region was demethylated (Figure 4A) as seen in RAG2-deficient pro-B cells (note that un-rearranged DFL16.1 and DSP regions in these cells represent fully germline IgH alleles as well as those in which a downstream DH gene segment has undergone recombination to form a DJH junction). However, DFL16.1/JH1 and DSP/JH1 recombined junctions were demethylated (Figures 4B and S4). For junctions that involved DFL16.1, demethylation of both parts of the recombination reaction was evident due to the presence of analyzable CpGs in each segment. Many DSP-involving junctions lacked CpG dinucleotides contributed from the DSP part. This is because all DSP gene segments lie within a 4-kb repeat unit ,, which is reflected in all DSP family members sharing a common CpG located 3′ of the gene segment. Two DSP gene segments, DSP2.2 and DSP2.3, have a CpG within the gene segment. In addition, the amplicon encompassing DSP2.3, includes a third CpG dinucleotide 5′ of the gene segment (Figure S3). The 3′ CpG is lost during recombination, and deletions that occur during recombination lead to many junctions that have no CpG contribution from the DH part (Figures 4B, 4C, and S4). Yet, demethylation of DSP/JH junctions was evident from the state of residual CpGs that fell within the JH1 region.
Figure 4. DNA methylation status after the first step of IgH locus recombination.
Pro-B cells were purified from the bone marrow of wild-type C57BL/6 mice. Data shown are derived from two independent preparations of pro- and pre-B cells obtained from six to eight mice in each experiment. This cell population contains a mix of germline and partially rearranged IgH alleles. After bisulfite modification, the genomic DNA was used to amplify unrearranged (A) and DJH rearranged junctions containing DFL16.1 and DSP gene segments (B). Cytosines derived from DH and JH gene segments are marked as circles and squares, respectively. Filled and open circles, or squares, indicate methylated and unmethylated cytosines, respectively. Numbers within regions marked as DFL16.1, DSP and JH1 in (B) denote CpG dinucleotides corresponding to the configuration of these residues at the respective unrearranged gene segments. For example, of the five CpGs at unrearranged DFL16.1 only the first two are retained in DFL16.1/JH1 junctions. The total numbers of CpG dinucleotides are reduced in junctional sequences because some residues are lost during VDJ recombination as described in the text. Additional heterogeneity is due to the imprecise nature of recombination. Pie charts summarize the percentage of methylated cytosines at each position, except where the number of alleles falls below 12 (indicated by asterisks). (C) Methylation state of recombined DJH alleles in purified pre-B cells. This population contains a mix of VDJH recombined and DJH recombined IgH alleles (see Figure 5A). Note that the number of circles and squares representing DH- or JH-associated CpGs differed in the DJH junctions compared to the corresponding unrearranged regions due to junctional variability.doi:10.1371/journal.pbio.1001475.g004
During B cell development, pro-B cells that contain a functional IgH allele undergo several rounds of cell division and differentiate into small pre-B cells . We found that pre-B cells also contained demethylated DFL16.1/JH1 and DSP/JH1 junctions (Figure 4C). Sequence diversity at recombination junctions provided an unequivocal measure of allelic individuality in our experiments. For example, many completely demethylated alleles, such as DFL16.1/JH1 junctions in pre-B cells, could have arisen by aberrant amplification of a single allele. However, analysis of the junctional sequences (Figure S4) showed that each of these represented unique alleles.
Demethylated DJH junctions could arise by preferential juxtaposition of demethylated DH and JH gene segments; alternatively, demethylation could be targeted to the junction after recombination. One prediction of allelic demethylation prior to recombination is that unrearranged DHs located 5′ of the DJH junction (see Figure 1) should be demethylated. To determine whether DNA demethylation was specific to the DJH junction, or occurred more widely over recombinant allele, we used mature B cells. Earlier studies have shown that about 40% of mature B cells from wild-type mice contain VDJH rearrangement on both alleles ,. In such cells, unrearranged DH gene segments are lost from the genome. The remainder contain one VDJH rearranged allele and one DJH rearranged allele; these cells therefore contain unrearranged DH gene segments 5′ to the rearranged DJH junction (Figure 5A). Mature B cells with germline IgH alleles have not been identified in normal mice. First, we determined the methylation state of the DJH junctions in mature B cells. As seen in pro- or pre- B cells, DFL16.1/JH1 and DSP/JH1 junctions were demethylated in mature B cells (Figure 5C). We then analyzed germline DFL16.1 in mature B cells to score for all alleles with downstream DH rearrangements. We also analyzed a region approximately 1.3 kb 5′ of DFL16.1 that would be intact on DFL16.1 rearranged alleles. We found that both these regions were hypermethylated in mature B cells approximating that seen in RAG2-deficient pro-B cells (Figure 5B). We conclude that demethylation of recombined alleles is highly localized to the DJH junction.
Figure 5. DNA methylation state of unrearranged and DJH recombined alleles in mature B cells.
(A) Mature B cells were purified from spleens of C57BL/6 mice, and the genomic DNA was subjected to bisulfite modification assays. 40% of these cells contain two VDJH recombined alleles and the remainder contains one VDJH and one DJH recombined allele. (B) Amplicons corresponding to unrearranged DFL16.1 gene segment and a region centered 1.3 kb 5′ to DFL16.1 were cloned and sequenced. For comparison, methylation of the same region in pro-B cells derived from RAG2-decificient bone marrow is shown in the bottom panel. Filled and open circles indicate methylated and unmethylated cytosines. Pie charts summarize the percentage of methylated cytosines at each position; data are derived from two independent spleen B cell preparations with two to four mice in each experiment. (C) DJH junctions were amplified from bisulfite modified DNA, followed by cloning and sequencing. Circles and squares represent cytosines from DH and JH1 gene segment, respectively. Filled and open circles, or squares, indicate methylated and unmethylated cytosines, respectively. Numbers within regions marked as DFL16.1, DSP, and JH1 denote CpG dinucleotides corresponding to the configuration at the respective unrearranged gene segments. For example, of the five CpGs at unrearranged DFL16.1, only the first two are retained in DFL16.1/JH1 junctions. Variations in the total number of cytosines are due to imprecise joining during VDJ recombination. Pie charts summarize the percentage of methylated cytosines. The asterisk indicates positions where less than 12 CpGs were observed due to reduced representation caused by junctional diversity.doi:10.1371/journal.pbio.1001475.g005
DNA Demethylation Requires the Intronic Enhancer Eμ
To understand the basis for localized demethylation, we considered the following possibilities. First, a specific DH gene segment and the JH region could be demethylated immediately before recombination. In this model demethylation would precede rearrangement and could possibly target the recombinase to a pre-selected DH gene segment. Second, demethylation may occur during recombination, after the DNA breaks have been introduced by recombinase. Third, demethylation of the DJH junction could occur after rearrangement. The last model predicts the existence of methylated DJH junctions that are somehow trapped between recombination and demethylation steps. As described below, we found two sources of such DJH recombined alleles.
The intronic enhancer Eμ controls key features of the IgH locus assembly via its effects on chromatin structure. Of note, Eμ-deleted IgH alleles undergo DH recombination but not VH recombination ,. We have recently found that the Eμ enhancer directs localized histone modification changes at DJH junctions and proposed that these changes configure the IgH locus for VH gene recombination . To determine whether Eμ affected the methylation status of the DJH junction, we applied the bisulfite procedure to genomic DNA of bone marrow pro-B cells from mice that lack a 220 nucleotide Eμ core . We first examined the two regulatory regions. Three of five CpG sites used to assess Eμ DNA methylation flank the core enhancer and are therefore present on the deleted alleles. These three sites were highly methylated on Eμ-deficient alleles (Figure 6A). CpGs associated with the juxtaposed loxP sites that substitute the Eμ core were also hypermethylated (Figure S5). Thus, a functional enhancer is required to demethylate this region. The JH1 region remained hypermethylated in Eμ− alleles, whereas the DQ52 region was unmethylated on Eμ− alleles indicating that its demethylation was Eμ-independent (Figure 6A).
Figure 6. DNA methylation state of IgH alleles in the absence of the intronic enhancer Eμ.
Pro-B cells were purified from the bone marrow of Eμ-deficient mice . Genomic DNA was subjected to bisulfite modification followed by PCR amplification of germline gene segments (A) and DJH junctions (B). The 220-bp deletion of Eμ is represented as partial blue arcs separated by a gap. Filled and open circles, or squares, indicate methylated and unmethylated cytosines, respectively. Numbers within regions marked as DFL16.1, DSP, and JH1 denote CpG dinucleotides corresponding to the configuration at the respective unrearranged gene segments. For example, of the five CpGs at unrearranged DFL16.1 only the first two are retained in DFL16.1/JH1 junctions. Three out of five cytosines used to analyze wild-type alleles remain in this deletion, and their methylation status is shown immediately below the disrupted enhancer. Sequences that substitute for the enhancer also contain CpGs whose methylation status is shown in Figure S5. (B) Recombined DFL16.1, DSP and DQ52 to JH1 junctions were amplified, cloned, and sequenced. Untemplated CpGs incorporated during VDJ recombination were found to be methylated (filled diamonds). Data shown are derived from two independent preparations of pro-B cells from Eμ-deleted mice, with four to six mice in each experiment.doi:10.1371/journal.pbio.1001475.g006
In striking contrast to our observations on Eμ-sufficient alleles, we found that DFL16.1/JH1and DSP/JH1 junctions were hypermethylated in Eμ-deficient pro-B cells (compare Figure 6B to Figures 4B, 4C, and 5C). Moreover, non-templated CpGs introduced by N region addition during recombination  were also methylated (Figure 6B, indicated by black diamonds). Interestingly, DQ52/JH1 junctions were of a hybrid nature, consisting of several demethylated CpGs from DQ52 and several methylated CpGs from the JH1 portions of the junction. We infer that demethylation at DJH junctions requires Eμ. The state of DQ52/JH1 junctions indicated that neither rearranging segment imposed its pattern of methylation during the “cut-and-paste” reaction of V(D)J recombination. These observations are consistent with a model where active demethylation follows recombination.
Junctional Demethylation Is Tissue Specific
DH to JH recombination does not occur exclusively in B lymphocyte precursors. In particular, DJH junctions have been detected in a significant proportion of DP thymocytes ,. To determine whether DJH junction demethylation was lineage restricted, we analyzed DNA from DP thymocytes from wild-type mice. We found that DQ52 and Eμ regions were demethylated, and germline DFL16.1 and JH regions were hypermethylated as seen in pro-B cells (Figure 7A). However, both DFL16.1/JH1 and DSP/JH1 junctions were considerably more methylated in DP cells than their state in pro-B cells (Figure 7B), and DQ52/JH1 junctions displayed the hybrid phenotype seen in Eμ-deficient pro-B cells. In these cases of methylated DJH junctions we again observed de novo methylation of non-templated N region CpGs (Figure 7B, indicated by black diamonds). Thus, despite the presence of an intact demethylated enhancer, DJH junctions in DP cells retained the methylation status of the rearranging gene segments. Our interpretation is that DH to JH recombination does not require prior DNA demethylation. After rearrangement, Eμ activity drives demethylation in pro-B cells; however, the milieu in DP thymocytes in terms of Eμ activity or availability of transcription factors, does not permit efficient junctional DJH demethylation.
Figure 7. DNA methylation of IgH alleles in thymocytes.
CD4+CD8+ thymocytes from C57BL/6 mice were enriched by adsorption to PNA-coated plastic plates. Genomic DNA purified from these cells was used in bisulfite modification analysis. Amplicons corresponding to unrearranged parts of IgH allele (A) and DJH junctions (B) were cloned and sequenced. Circles and squares depict CpGs corresponding to DH and JH1 cytosines, respectively. Filled circles/squares correspond to methylated cytosines; pie charts summarize the percentage of methylated cytosines at each position except where the number of sequenced alleles falls below 12, indicated by asterisks. Untemplated CpGs incorporated during VDJ recombination were found to be methylated (filled diamonds). Data shown were obtained from two independent preparations of thymocytes, using one mouse per experiment.doi:10.1371/journal.pbio.1001475.g007
Systematic analysis of DNA methylation of unrearranged and partially rearranged IgH alleles in primary cells revealed several interesting features. First, the lack of correlation between histone modifications and DNA methylation status indicates that these marks are independently regulated. This was most clearly evident in the JH region that is marked with the highest levels of histone acetylation and H3K4me3 in the unrearranged state, yet remains hypermethylated. Furthermore, germline DFL16.1 and the DSP gene segments were comparably methylated despite being differentially marked at the level of histone modifications. These observations appear to be at odds with genomic studies that show a correlation between histone acetylation and DNA demethylation, and H3K9 methylation and DNA methylation ,,. We suggest that the results from genome-wide studies may be skewed towards promoter-based CGIs. Instead, the sites we analyzed were largely non-CGIs and included enhancers (such as Eμ), promoters (such as DQ52), cryptic promoters (such as DSP and DFL16.1, see below), and regions that cannot be categorized as any of these.
An important corollary of this observation is that pre-rearrangement DNA methylation status correlates poorly with recombination potential. Recent studies identified the JH region as a RAG1/2-rich recombination center . Our observation suggests that DNA demethylation is not required to generate the recombination center. Additionally, DFL16.1 and DQ52 gene segments that rearrange most frequently – have very different levels of DNA methylation prior to rearrangement; conversely, DSP gene segments that rearrange relatively less frequently have comparable levels of DNA methylation as DFL16.1. Taken together, our working model is that DNA methylation status does not guide the first step of IgH gene assembly.
We note, however, that we cannot unequivocally rule out the possibility that selective demethylation of specific DH and JH segments occur in a subset of cells just prior to recombination. For example, while the JH1 region that we analyzed was largely methylated in pro-B cells prior to rearrangement, we observed that a minority of alleles were demethylated in both RAG-sufficient and RAG-deficient pro-B cells. These demethylated JH1 alleles may represent a subset of cells in which DH recombination will occur preferentially to the JH1 gene segment. It will be interesting to determine whether other JH gene segments are similarly singled out for demethylation in subsets of pro-B cells.
Second, sites of maximal tissue-specific CpG demethylation in the germline IgH locus corresponded to the two strongest DNase 1 hypersensitive sites in the 70-kb DH–Cμ domain, DQ52 and Eμ. Because these regions share transcriptional regulatory properties, it is likely that their demethylation is linked to transcription or transcription-associated chromatin changes. Additionally, we noted highly selective CpG demethylation of some sequences and partial demethylation of others 5′ of DFL16.1 where three additional DNase 1 HSs have been recently identified. Though these observations link DNase 1 hypersensitivity to DNA methylation, this doesn't seem to be always the case. For example, JH region is known to be highly sensitive to DNase 1 digestion  (without being associated with “classical” DNase 1 hypersensitive sites), yet it is not demethylated. A possible model for this pattern of methylation emerges from the recent demonstration that Eμ is in spatial proximity to the cluster of DHSs 5′ of DFL16.1 ,,. Since Eμ itself has a demethylating potential (Eμ flanking regions are methylated on Eμ-deficient alleles), perhaps Eμ looping to 5′ DFL16.1 sites “delivers” demethylating activities to this region. The partial demethylation observed at 5′ DFL16.1 sites may reflect that such loops are present in only a fraction of pro-B cells at any given time. Consistent with the idea that Eμ induces demethylation in a subset of cells, the form of partial demethylation seen at DFL16.1 (−6.5) involves extensive demethylation of some alleles and essentially complete methylation of others (Figure 3). Eμ has also been proposed to loop to DHS5-7 of the 3′ regulatory region at the 3′ of the IgH locus. Interestingly, earlier studies by Giambra et al.  showed that DHS5-7 was partially demethylated in a pro-B cell line in a manner similar to the methylation state 5′ of DFL16.1. This pattern may be the consequence of Eμ looping to DHS5-7 in a subset of pro-B cells. Overall, we propose that the pattern of DNA demethylation in the germline IgH locus is determined by spatial proximity to Eμ.
Third, we found that DJH junctions were extensively demethylated. Though we cannot discount the possibility that germline DH and JH gene segments were demethylated just before recombination, we favor the hypothesis that demethylation of DJH junction occurred after recombination. The reasons for this are two-fold: first, DJH junctions remain methylated in Eμ-deficient pro-B cells and in CD4+CD8+ thymocytes. Our interpretation is that these DJH alleles are caught in an intermediate stage where recombination has occurred but demethylation has not. Second, we think it is unlikely that demethylation occurred prior to recombination followed by re-methylation of DJH junctions based on the state of DQ52/JH1 junctions. In both Eμ-deficient pro-B cells and DP thymocytes the methylation status of DQ52/JH1 junctions was such that the DQ52 portion was demethylated and the JH1 portion was methylated. If demethylation preceded rearrangement, generation of each hybrid junction would require re-methylation of a subset of closely associated CpGs.
Though our data clearly demonstrate that DNA demethylation of associated gene segments is not essential for DH to JH recombination, we note that the two instances of methylated DJH junctions identified in this study involve circumstances where the frequency of DH recombination is lower than in wild-type pro-B cells. DH recombination has been estimated to be 5- to 10-fold lower in Eμ-deleted pro-B cells  and only about 30%–50% of IgH alleles in DP thymocytes  have DJH junctions. We cannot rule out, therefore, that gene segment demethylation may increase the efficiency of DH recombination in wild-type pro-B cells. Because Eμ deletion also leads to loss of other accessibility-associated epigenetic marks in the unrearranged locus, it is difficult to deconvolute the contribution of each mark to recombination efficiency.
How might DNA demethylation be targeted to DJH junctions? We have previously shown that DH recombination activates cryptic bi-directional promoters associated with most DH gene segments . Transcriptional activity and associated RNA polymerase II recruitment is restricted to the DJH junctions, and drops off before the unrearranged DH segments located 5′ of the junction. These changes require Eμ and the simplest interpretation is that recombination places cryptic DH promoters under the influence of Eμ. Our working model is that DH-promoter/Eμ interaction brings Eμ-associated demethylating activity to DJH junctions, thereby resulting in Eμ-directed demethylation. Because Eμ's influence does not extend to the next upstream DH gene segment, DJH demethylation is highly localized. In this model the substantial demethylation of DJH junctions compared to partial demethylation of looping sites may be due to stronger interaction of Eμ with DJH promoters compared to Eμ interaction with the 3′ RR or 5′ of DFL16.1 sequences.
Does DJH demethylation serve a function? In this regard it is interesting to note that VH recombination is significantly reduced in both instances where DJH junctions remain methylated (Eμ-deleted alleles and in DP thymocytes). Though this is consistent with the view that DJH demethylation facilitates VH recombination, we think that the regulation of VH recombination is more complex. The highly localized Eμ-dependent DJH demethylation that we describe in this report adds to the emerging evidence that DJH junctions are distinguished from un-rearranged DH gene segments by several forms of epigenetic changes . These include activation-associated histone modifications, such as H3 acetylation and H3K4me3 and increased sensitivity to DNase I. Like DJH demethylation, these alterations are also restricted to DJH junctions and are Eμ dependent. Taken together, our working model is that all these DJH-restricted epigenetic changes work in concert to promote the timing and precision of VH recombination.
Materials and Methods
Rag2-deficient and C57BL/6 mice were obtained from Jackson Laboratory (Bar Harbor, Maine) and housed in pathogen-free facilities at the NIA or at the University of Massachusetts Medical School. Eμ-deficient mice were generated and maintained in the facilities at Childrens Hospital (Boston, Massachusetts). Mouse experiments were approved by the Animal Care and Use Committees at the NIA, Harvard Medical School, and the University of Massachusetts Medical School.
A magnetic cell-sorting system was used to purify pro-B and mature B cells as previously described . Briefly, to obtain pro-B cells with IgH in germline configuration bone marrow cells were recovered from 6–8-wk-old RAG2-deficient mice by flushing the femur and tibia with 10% calf serum in PBS. CD19+ cells were purified from single cell suspension of bone marrow by positive selection using paramagnetic microbeads (Miltenyi Biotech). To obtain mature B cells, the same procedure was followed using cells obtained from the spleen of C57BL/6 mice. The resulting cells were greater than 90% CD19+ by flow cytometry.
FACS Sorting of Pre-B Cells and Pro-B cells from C57BL/6 Mice
Freshly isolated bone marrow cells were re-suspended to 6×107/ml in staining media containing biotin-, flavin-, and phenol red-deficient RPMI 1640 (Irvine Scientific), 10 mM Hepes, pH 7.2, 0.02% sodium azide, 1 mM EDTA, and 3% newborn calf serum, and treated with Fc block for 10 min on ice. Cells were incubated with primary antibodies for 20 min and then washed three times, incubated with SA-Cy5PE for 15 min, and then washed three more times. After the final wash, samples were re-suspended in 1 µg/ml propidium iodide to exclude dead cells. Primary antibodies included B220 APC; CD43 (clone S7) PE; IgM FITC; Ly6C FITC; BP-1 (clone 6C3) biotin. Antibodies were purchased from BD Biosciences/Pharmingen, eBioscience, or CALTAG. FACS Sorting was performed on a 3-laser, 9-detector MoFlo. Data were analyzed for presentation purposes with FlowJo software (Tree Star).
DP thymocytes were purified by PNA agglutination. In brief, equal volumes of cell suspension (7–8×108 cells/ml) and PNA solution (1.5 mg/ml) were incubated for 1 h at 4°C, followed by sedimentation in fetal calf serum (Invitrogen). PNA-agglutinated cell pellet was dissociated by D-galactose treatment resulting in greater than 90% pure CD4+CD8+ cells.
Genomic DNA Isolation and Modification
Genomic DNA was extracted from 1–5×106 cells by using the DNeasy Blood & Tissue Kit (QIAGEN) according to the manufacturer's protocol.
Sodium bisulfite modification was carried out as described earlier  with some modifications. Briefly, 1 µg of genomic DNA was denatured by incubating with NaOH at 37°C for 15 min. Sodium metabisulfite (3 M) and hydriquinone (0.5 mM) were added to the samples together and incubated overnight at 55°C. The reaction was stopped by incubating samples with NaOH 15 min at 37°C. Modified DNA was purified using GeneClean II (MP Biomedicals). Modified DNA was precipitated and dissolved in 30 µl of 1 mM Tris-HCl (pH 8.0).
Modified templates were amplified by nested PCR using primers listed in Table S2. PCR products were separated by agarose gel electrophoresis, purified using QIAquick gel extraction kit (QIAGEN), and cloned into pCRII-TOPO vector. Mini prep DNA containing amplicon inserts were identified by PCR and sequenced commercially (SeqWright). Sequence analysis showed 99%–100% bisulfite modification efficiency (Figure S6).
Comparison of DNA methylation status between two independent experiments. For each region analyzed in the paper, we used at least two independent DNA preparations starting with cells obtained from six mice for each sample. Methylation profiles of the regions amplified from two independent experiments were comparable, as shown with the example of the DFL16.1 region in RAG−/− pro-B mice.
DNA methylation state of the mb-1 and β-globin locus activating region in different cell types. For our experiments we have chosen the previously characterized mb-1 gene and its promoter as a positive and β-globin locus activating region as a negative control. Sequences from at least 12 individual colonies were analyzed for each cell type. Consistent with the observations of the Hagman group  mb-1 gene and its promoter are hypomethylated in early stages of B cell development. As expected mb-1 and its promoter were methylated in CD4+CD8+ DP thymocytes and kidney cells, which served as a negative control for this region. β-globin locus activating region was methylated in a RAG2-deficient pro-B cell line, primary RAG2-deficient pro-B cells, and kidney.
Relative positions of the CpG dinucleotides in the amplicons analyzed. Amplicons covering 11 regions of the germline IgH locus are depicted. Numbers in circles represent the order of the CpGs in each amplicon. DH gene segments are highlighted in green, JH1 region is highlighted in orange. Recombination signal sequences (RSSs) with 12- and 23-bp spacers are highlighted in light blue and grey, respectively. Distances (in nucleotides) between CpG dinucleotides and gene segments are indicated.
Analysis of individual sequences at DJH junctions. While analyzing DNA methylation profiles, one question arises especially in the case of demethylated sequences: whether this is a single DNA molecule having been amplified during PCR. To answer that question we have performed a careful analysis of DNA at the joining regions; nucleotides introduced by non-templated end-joining permit unequivocal assignment of sequences to individual alleles. Aligned sequences of a representative cell type, pre-B cells, are shown here.
DNA methylation state of the Eμ region in Eμ− pro-B cells. The schematic representation of the Eμ region in Eμ− cells is represented on top. CpGs are depicted as vertical bars; those CpGs that correspond to the ones in the wild-type sequence adjacent to the Eμ deletion sites, are depicted in blue color, the ones that are introduced during cre-deletion in brown.
Bisulfite modification efficiency. We compared each modified sequence with the genomic DNA sequence to determine the efficiency of cytosine conversion. Sequences used in the analysis showed 99%–100% modification efficiency.
Summary of bisulfite modification analysis. Number of sequences analyzed for each amplicon (gene segment) in each cell type (cell type) are indicated (number of sequences).
Primer sequences used to amplify indicated amplicons for bisulfite modification analysis.
We thank Ursula Storb, Jim Hagman, and Steve Smale for comments on the manuscript and Susanne Feehley and Valerie Martin for editorial assistance.
The author(s) have made the following declarations about their contributions: Conceived and designed the experiments: RSelimyan, RSen. Performed the experiments: RSelimyan, II, PP. Analyzed the data: RSelimyan, RSen. Contributed reagents/materials/analysis tools: RMG, RSubrahmanyam, TP, FWA. Wrote the paper: RSelimyan, RSen.
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