Circadian timing is generated through a unique series of autoregulatory interactions termed the molecular clock. Behavioral rhythms subject to the molecular clock are well characterized. We demonstrate a role for Bmal1 and Clock in the regulation of glucose homeostasis. Inactivation of the known clock components Bmal1 (Mop3) and Clock suppress the diurnal variation in glucose and triglycerides. Gluconeogenesis is abolished by deletion of Bmal1 and is depressed in Clock mutants, but the counterregulatory response of corticosterone and glucagon to insulin-induced hypoglycaemia is retained. Furthermore, a high-fat diet modulates carbohydrate metabolism by amplifying circadian variation in glucose tolerance and insulin sensitivity, and mutation of Clock restores the chow-fed phenotype. Bmal1 and Clock, genes that function in the core molecular clock, exert profound control over recovery from insulin-induced hypoglycaemia. Furthermore, asynchronous dietary cues may modify glucose homeostasis via their interactions with peripheral molecular clocks.
Citation: Rudic RD, McNamara P, Curtis A-M, Boston RC, Panda S, et al. (2004) BMAL1 and CLOCK, Two Essential Components of the Circadian Clock, Are Involved in Glucose Homeostasis. PLoS Biol 2(11): e377. doi:10.1371/journal.pbio.0020377
Academic Editor: Steve O'Rahilly, University of Cambridge
Received: February 11, 2004; Accepted: August 31, 2004; Published: November 2, 2004
Copyright: © 2004 Rudic et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Competing interests: The authors have declared that no conflicts of interest exist.
Abbreviations: Bmal1 −/− , Bmal knockout mice; Clockmut , Clock mutant mice; CT[number], circadian time [designated time point]; FSIGT, frequent sampling intravenous glucose tolerance test; GT, glucose tolerance; HF, high-fat diet; IT, insulin tolerance; PEPCK, phosphoenolpyruvate carboxykinase; RC, regular chow diet; SCN, suprachiasmatic nucleus; Sg, glucose-mediated glucose disposal; Si, insulin sensitivity; WT, wild-type
The master clock, which, in mammals, resides in the hypothalamic suprachiasmatic nucleus (SCN), is thought to synchronize multiple peripheral oscillators to ensure temporal coordination of behavior and metabolism. Peripheral clocks amplify or dampen central rhythms or exhibit autonomous behavior to facilitate local adaptive responses (Hastings et al. 2003). The central clock may communicate to modulate or entrain rhythms in the periphery via hormones (McNamara et al. 2001) or hemodynamic cues. Asynchronous environmental cues, such as eating, also influence the autonomous behavior of peripheral clocks (Damiola et al. 2000; Stokkan et al. 2001).
The variation in sleep and wakefulness (activity) is perhaps the most well-known circadian rhythm. Surgical ablation of the SCN in mice (Ibuka et al. 1980; Welsh et al. 1988) and rats (Ibuka et al. 1977; Mosko and Moore 1979) abolishes the nocturnal burst in locomotor activity. Similarly, disruption and/or mutation of Bmal1 (Bunger et al. 2000) or Clock (Vitaterna et al. 1994), transcription factors that compose the positive limb of an autoregulatory feedback loop in the core molecular clock (Young and Kay 2001; Reppert and Weaver 2002), also impairs circadian behavior. Bmal1 and Clock may influence behavioral rhythms by regulating the firing rate of SCN neurons (Herzog et al. 1998; Deboer et al. 2003).
Genes relevant to the molecular clock are also expressed in peripheral tissues (Akhtar et al. 2002; Kita et al. 2002; Panda et al. 2002; Storch et al. 2002; Oishi et al. 2003) where approximately 5%–10% of the transcriptome is subject to circadian oscillation (Albrecht and Eichele 2003). Although the precise role of peripheral clocks and the mechanisms that link them to the SCN remain largely obscure, genetic mutation or deletion has implicated peripheral clocks in the regulation of some aspects of cellular function, including division (Matsuo et al. 2003), estrous cyclicity (Miller et al. 2004), and phospholipid metabolism (Marquez et al. 2004). Glucose and lipid homeostasis are also known to exhibit circadian variation (Seaman et al. 1965; Malherbe et al. 1969; Gagliardino and Hernandez 1971; Schlierf and Dorow 1973). Surgical ablation of the SCN impairs the control of glucose homeostasis (la Fleur et al. 2001). However, the proximity of satiety centres to the SCN has potentially confounded interpretation of these results. Indeed, there is no direct evidence implicating the molecular clock in the regulation of glycaemia or insulin sensitivity (Si).
Our studies revealed a profound role for core clock genes—Bmal1 and Clock—in regulating recovery from insulin-induced hypoglycaemia. Furthermore, the impact of a high-fat diet (HF) was to amplify the diurnal variation in glucose tolerance and Si in a manner dependent on the Clock gene. These studies suggest that the temporal distribution of a caloric load may influence the response to insulin and that circadian variability in glucose homeostasis may be subject to modulation by asynchronous dietary cues.
We examined the role of the molecular clock in glucose homeostasis by using mice in which core clock genes are impaired (Clockmut) or deficient (Bmal1−/−). Both plasma glucose and triglycerides were subject to circadian variation in wild-type (WT) mice, peaking at approximately circadian time point 4 (CT4) and CT28 (where CT0 is subjective day beginning at 7 AM, and CT12 is subjective night beginning at 7 PM) (Figure 1A and 1B), as reported previously (Seaman et al. 1965; Schlierf and Dorow 1973). We also observed that corticosterone (Figure 1C), which stimulates gluconeogenesis during hypoglycaemia (Cryer 1993), and adiponectin (Figure 1D), which has been associated with insulin resistance (Yamauchi et al. 2001; Maeda et al. 2002), oscillated significantly, but out of phase with the glucose and triglyceride rhythms. Diurnal variation in glucose and triglycerides, but not in corticosterone, was disrupted in the mutant mice (Table 1).
Figure 1. Circadian Variation of Glucose, Triglyceride, and Hormone Levels in Circulating Blood
Plasma from whole blood isolated from unchallenged WT mice at different CTs was analyzed for glucose (A), triglyceride (B), corticosterone (C), and adiponectin levels (D) (n = 12 per time point). Results for Bmal1−/− and Clockmut mice are shown in Table 1.doi:10.1371/journal.pbio.0020377.g001
Table 1. Clock-Controlled Metabolic Rhythmsdoi:10.1371/journal.pbio.0020377.t001
Although there was no clear rhythm in the hypoglyacemic response to insulin, recovery of blood glucose exhibited a robust circadian variation (Figure 2A), with an excessive rebound from the effects of insulin evident at subjective dawn (CT19 and CT25) (Figure 2A). Insulin caused a profound hypoglyacemic response, independent of clock time, in both Bmal1−/− and Clockmut mice (Figure 2B). This response was more pronounced in the former, consistent with the comparative severity of the molecular and behavioral phenotypes between the Bmal1−/− and Clockmut animals (King et al. 1997; Bunger et al. 2000). Despite exacerbation of the hypoglycaemic response to insulin in the mutants, the counterregulatory responses of both corticosterone and glucagon were retained (Figure 2C and 2D).
Figure 2. Disruption of Genes in the Core Molecular Clock Alters the Response to Insulin
(A) Insulin tolerance (IT) was examined in WT mice on CT7, CT13, CT19, and CT25 at 30 min, 60 min, and 90 min after insulin injection (n = 12 per time point, *p < 0.01).
(B) IT was examined in WT (black line), Bmal1−/− (blue line), and Clockmut mice (green line) at CT1 (i) and CT13 (ii) (n = 6–10, *p < 0.05, †p < 0.01, ††p < 0.001).
(C and D) Plasma levels of the counterregulatory hormones corticosterone (C) and glucagon (D) were assessed 60 min after insulin injection in Bmal1−/− and Clockmut mice (n = 7, corticosterone assay; samples were pooled for glucagon assay, *p < 0.05).doi:10.1371/journal.pbio.0020377.g002
Gluconeogenesis also contributes to restoration of blood glucose after insulin-induced hypoglycaemia. Consistent with this observation, conversion of exogenously administered pyruvate to glucose, which reflects gluconeogenesis (Miyake et al. 2002), was impaired in the Clockmut animals. This impairment was most marked in Bmal1−/− mice, while Bmal1+/− and Clockmut mice exhibited an intermediate phenotype when compared with WT littermate controls (Figure 3A). Furthermore, activity of the key rate-limiting enzyme of gluconeogenesis, phosphoenolpyruvate carboxykinase (PEPCK), exhibited diurnal variation in the liver and aorta that was blunted in Clockmut mice (Figure 3B). PEPCK activity in kidney was antiphasic to the rhythm in aorta and liver and was unimpaired in Clockmut mice (Figure 3B), suggesting tissue-specific regulation of enzyme activity.
Figure 3. Impaired Gluconeogenesis in Mice with a Disrupted Circadian Clock
(A) Pyruvate tolerance was compared among WT (black line), Bmal1+/− (blue line), and Bmal1−/− (purple line), and Clockmut (green line) mice at CT7 (n = 6–10).
(B) Relative PEPCK activity (units are expressed as luciferase activity × 103) was measured in liver (i), aorta (ii), and kidney (iii) from WT (white bars) and Clockmut mice (green bars).doi:10.1371/journal.pbio.0020377.g003
The frequent sampling intravenous glucose tolerance test (FSIGT) was performed to assess more precisely the impact of the molecular clock on sensitivity to insulin. This test provides an estimate of Si, consistent with that obtained by the euglycaemic clamp (Pacini et al. 2001). Additionally, data modeling provides estimates of glucose-mediated glucose disposal (Sg), insulin secretion, and Si. Si and insulin secretion, but not Sg, exhibited a diurnal variation in WT mice fed a regular chow diet (RC) (Table 2). Circadian variation of glucose and lipid homeostasis might condition the metabolic response to asynchronous environmental cues, such as diet, that impinge on Si. Dyslipidemia coincides with insulin resistance in the metabolic syndrome (Brotman and Girod 2002), and a diet high in fat impairs Si (Grundleger and Thenen 1982; Coulston et al. 1983). Both HF-fed WT and HF-fed Clockmut mice increased body weight significantly and to a similar degree in comparison to their age-matched, RC-fed controls (Table 3). Body fat composition averaged 17.6% of lean body mass in RC-fed WT mice, rising to 27.7% (p < 0.002) on high-fat feeding. Again, fat composition was not significantly altered by the presence of the Clock mutation (Table 3).
Table 2. Indices of Noninsulin- and Insulin-Mediated Parameters of Glucose Disposal Derived from Modelling of FSIGT Data at CT1 and CT13doi:10.1371/journal.pbio.0020377.t002
Table 3. Body Mass Index Was Assessed in Mice Feeding on RC and HFdoi:10.1371/journal.pbio.0020377.t003
Glucose tolerance on an RC trended towards an intolerant phenotype at CT1 versus CT13, but this difference did not attain significance (Figure 4A). This is consistent with the temporal variation in insulin secretion observed in RC-fed WT mice in the FSIGT experiment (Table 2). However, when the mice were fed HF for 2 mo, this glycaemic excursion at CT1 evoked by the environmental challenge was amplified and significant (two way analysis of variance [ANOVA]; F = 63.2, p < 0.001) (Figure 4A). Similarly, although the hypoglycaemic response to insulin was not different in mice fed regular chow at CT1 and CT13 (Figure 4B), the HF induced a significant temporal variation (Figure 4B). Thus, the impact of a high fat intake on carbohydrate metabolism in WTs includes an amplification of the diurnal variation in the response to both glucose and insulin. This coincides with a modest impairment in the ability to restore euglycaemia after insulin. A similar impairment resulting from a defect in gluconeogenesis has been observed in rats (Oakes et al. 1997). The mutants failed to exhibit a significant time-dependent variation in their response to glucose or insulin, again reminiscent of the RC-fed, WT phenotype (Figure 4).
Figure 4. The Molecular Clock Conditions HF-Induced Circadian Variation in Glucose Homeostasis
(A) Glucose tolerance (GT) in RC-fed WT mice (i), HF-fed WT mice (ii), and HF-fed Clockmut mice (iii).
(B) IT in RC-fed WT mice (i), HF-fed WT mice (ii), and HF-fed Clockmut mice (iii) at respective times (n = 6–8; *p < 0.05, †p < 0.01).doi:10.1371/journal.pbio.0020377.g004
Mice were also subjected to extended high-fat feeding (11 mo versus 2 mo). Long-term hyperlipidemia is known to induce frank diabetes with impaired release of insulin (Johnson et al. 1990), in contrast to short-term, high-fat feeding, which increases release of insulin, but impairs the response to it (Linn et al. 1999). The extended HF impaired insulin secretion, reflected by its marked reduction to negative values in HF-fed WT mice (Table 2). The lack of insulin secretion resulted in calculated values of Si that were imperceptibly low (see Material and Methods). However, Sg, insulin secretion, and Si were restored to a WT phenotype in Clockmut mice that were also HF-fed for 11 mo (Table 2). This is, again, consistent with a role for the molecular clock in conditioning the response of glucose metabolism to the intake of dietary fat. Remarkably, mutation of the molecular clock protected against the development of frank diabetes caused by chronic high-fat feeding.
Maintenance of blood glucose levels within a narrow range is critical to mammalian survival, and environmental cues can trigger appropriate tissue disposition of glucose through adaptive behaviors such as in hibernation (Castex and Hoo-Paris 1987) or the “fight-or-flight” response (Surwit et al. 1992). In this sense, glucose regulation is a fundamental and ancestral defence mechanism. Our studies suggest that Bmal1 and Clock, core components of the molecular clock, contribute substantially to regulation of recovery from the hypoglycaemic response to insulin. However, other mechanisms also impinge on this ancient adaptive response. Thus, impaired recovery from insulin-induced hypoglycaemia is observed in mice lacking proopiomelanocortin. These animals lack adrenal glands and melanocortins and exhibit a defective glucagon response to insulin-induced hypoglycaemia (Hochgeschwender et al. 2003). They contrast with the Bmal1−/− and Clockmut mice, where the counterregulatory hormone response is unimpaired. Thus, steroids, epinephrine, and glucagon appear to facilitate recovery from insulin-induced hypoglycaemia in a manner distinct from, but complementary to, the molecular clock.
An assumption intrinsic to our studies is that the phenotypes revealed in the mutant mice are attributable to their function as core elements of the molecular clock. However, as trans-activators, both Clock and Bmal1 may have pleiotropic effects independent of the circadian clock that could impinge on metabolism. Several lines of evidence argue against this hypothesis. First, genes relevant to these metabolic phenotypes display circadian oscillations in their steady-state mRNA levels (Young et al. 2001; Oishi et al. 2003). In addition, the mRNA levels of many of these key proteins are phase-aligned with Per1 (e.g., Enolase 3, Pgam, Transketolase, Lipase, Lpl, Dgat1, Ppar alpha) or Per2 (e.g., Mod1, Lpl, Pepck, lipin 1) (unpublished data). In addition, many of their mRNAs are at lower levels in Bmal1−/− (e.g., Mod1, Pepck, Enolase3, Pgam) (unpublished data), consistent with a direct role of Bmal1 in their transcription. Thirdly, these metabolic parameters are disrupted in both circadian mutants with the same rank order of potency as the locomotor activity phenotypes (Bmal1−/− > Clockmut). Thus, the most parsimonious interpretation is that the observed metabolic deficiencies in the Bmal1−/− and Clockmut mice are due to their roles in the circadian clock, rather than to “off-clock” effects.
We observed that the impact of HF on glucose homeostasis was apparently to emphasize the role of the molecular clock. Diet has previously been shown to interact with peripheral clocks. Changes in feeding shift the circadian pattern of gene expression in the liver, but not in the master clock in the SCN (Damiola et al. 2000), demonstrating the importance of food as a cue to circadian control. Individual constituents of food could also provide discrete stimuli. For example, glucose alone can induce rhythmic gene expression in isolated fibroblasts (Hirota et al. 2002). Thus, dietary composition, the size and timing of a feed might all be expected to interact differentially with an underlying circadian regulation of metabolic control.
Alterations in dietary content, the availability of “fast food,” inactivity, and sociocultural factors have all been implicated in the emergence of the metabolic syndrome as a major challenge to the public health (Zimmet et al. 2001). However, while mechanistic integration of the diverse elements of the syndrome has proven elusive, our studies suggest that timing may influence the functional consequences of ingesting a caloric load.
Materials and Methods
Mice were acclimatized for 2 wk in 12 h light–12 h dark cycles before being subjected to a 36-h period of constant darkness followed by experimentation in darkness. Experimental chronology is measured in CT, subjective day beginning at 7 AM (CT0), and subjective night beginning at 7 PM (CT12).
WT and Clockmut mice were placed on an HF (Teklad, TD02435) and compared to age-matched WT mice on a regular chow diet (RC). Mice were on RC for 8 wk except for those subjected to FSIGT where they received RC for 11 mo. Body mass composition was measured by dual energy X-ray absorptiometry at 10 mo.
Tests were performed as described (Klaman et al. 2000) with a diminution in the glucose bolus (0.1 g/kg).
Intravenous glucose tolerance test and minimal modeling.
The tolerance test was performed as described (Pacini et al. 2001) in unanesthetized mice, and the minimal model of Bergman et al. (1979) was applied to the data using MINMOD software (Boston et al. 2003). The derived values were Si, Sg, and acute insulin response to glucose, which measures insulin secretion. Si is the ratio of insulin delivery rate to the interstitium to insulin extraction rate from the interstitium. Long-term feeding of HF to WT mice resulted in imperceptibly small insulin sensitivity values. This could be the consequence of impaired delivery of insulin to the interstitium, exacerbated extraction rate, or a combination of both factors. Insulin secretion is derived from area under the insulin curve, above basal, from 0 to 10 min after glucose infusion; and disposition index, which equals the product of insulin sensitivity multiplied by insulin secretion and measures the degree to which insulin sensitivity can be compensated for by elevated insulin secretion (Pacini et al. 2001).
Insulin, leptin, corticosterone, and glucagon levels were measured by immunoassays from Crystalchem (Downers Grove, Illinois, United States), ICN Biochemicals (Costa Mesa, California, United States), and Linco Research (St. Charles, Missouri, United States). Plasma glucose was measured by the glucose oxidase method using a glucose analyzer machine for FSIGT and by glucometer for the intraperitoneal tolerance test. PEPCK activity was quantitated by a bioluminescent method (Wimmer 1988).
The significance of differences amongst the tolerance test curves was assessed by distribution-free two-way ANOVA with a Bonferroni correction. FSIGT data were tested by one-way ANOVA with the Kruskal-Wallis test. Paired Student's t-tests were used to perform comparisons of corticosterone levels before and after insulin injection in Bmal−/− mice and between WT and Clockmut mice. Plasma samples for glucagon analysis were pooled and were thus not compared by a formal statistical analysis. Results are presented as mean ± standard error of the mean (SEM), except for the FSIGT data (Table 2), presented as mean ± fractional standard deviation. Differences were considered significant when p < 0.05.
We wish to thank Christopher A. Bradfield and Celeste Simon for graciously providing MOP3 knockout mice and Joseph S. Takahashi and Amita Sehgal for providing Clock mutant mice. We also thank the Penn Diabetes Center/Mouse Phenotyping, Physiology, and Metabolism Core (NIH P30DK19525). We are indebted to Daniel J. Rader, Karen Teff, and Mitch Lazar for their helpful suggestions. Finally, we wish to thank Darko Stefanovski, Margaret Lucitt, and Eileen Clark for technical assistance. Supported in part by grants from the National Institutes of Health (HL 62250 and HL 54500) and fellowship grants from the American Heart Association to RDR (0225478U) and to PM (0160504U). GAF is the Robinette Foundation Professor of Cardiovascular Medicine and the Elmer Bobst Professor of Pharmacology.
RDR, PM, and GAF conceived and designed the experiments. RDR, PM, AMC, and SP performed the experiments. RDR, RB, and JH analyzed the data. RB and JH contributed reagents/materials/analysis tools. RDR wrote the paper.
- 1. Akhtar RA, Reddy AB, Maywood ES, Clayton JD, King VM, et al. (2002) Circadian cycling of the mouse liver transcriptome, as revealed by cDNA microarray, is driven by the suprachiasmatic nucleus. Curr Biol 12: 540–550.
- 2. Albrecht U, Eichele G (2003) The mammalian circadian clock. Curr Opin Genet Dev 13: 271–277.
- 3. Bergman RN, Ider YZ, Bowden CR, Cobelli C (1979) Quantitative estimation of insulin sensitivity. Am J Physiol 236: E667–677.
- 4. Boston RC, Stefanovski D, Moate PJ, Sumner AE, Watanabe RM, et al. (2003) MINMOD millennium: A computer program to calculate glucose effectiveness and insulin sensitivity from the frequently sampled intravenous glucose tolerance test. Diabetes Technol Ther 5: 1003–1015.
- 5. Brotman DJ, Girod JP (2002) The metabolic syndrome: A tug-of-war with no winner. Cleve Clin J Med 69: 990–994.
- 6. Bunger MK, Wilsbacher LD, Moran SM, Clendenin C, Radcliffe LA, et al. (2000) Mop3 is an essential component of the master circadian pacemaker in mammals. Cell 103: 1009–1017.
- 7. Castex C, Hoo-Paris R (1987) Regulation of endocrine pancreas secretions (insulin and glucagon) during the periodic lethargy-waking cycle of the hibernating mammal. Diabete Metab 13: 176–181.
- 8. Coulston AM, Liu GC, Reaven GM (1983) Plasma glucose, insulin and lipid responses to high-carbohydrate low-fat diets in normal humans. Metabolism 32: 52–56.
- 9. Cryer PE (1993) Glucose counterregulation: Prevention and correction of hypoglycemia in humans. Am J Physiol 264: E149–155.
- 10. Damiola F, Le Minh N, Preitner N, Kornmann B, Fleury-Olela F, et al. (2000) Restricted feeding uncouples circadian oscillators in peripheral tissues from the central pacemaker in the suprachiasmatic nucleus. Genes Dev 14: 2950–2961.
- 11. Deboer T, Vansteensel MJ, Detari L, Meijer JH (2003) Sleep states alter activity of suprachiasmatic nucleus neurons. Nat Neurosci 6: 1086–1090.
- 12. Gagliardino JJ, Hernandez RE (1971) Circadian variation of the serum glucose and immunoreactive insulin levels. Endocrinology 88: 1532–1534.
- 13. Grundleger ML, Thenen SW (1982) Decreased insulin binding, glucose transport, and glucose metabolism in soleus muscle of rats fed a high fat diet. Diabetes 31: 232–237.
- 14. Hastings MH, Reddy AB, Maywood ES (2003) A clockwork web: Circadian timing in brain and periphery, in health and disease. Nat Rev Neurosci 4: 649–661.
- 15. Herzog ED, Takahashi JS, Block GD (1998) Clock controls circadian period in isolated suprachiasmatic nucleus neurons. Nat Neurosci 1: 708–713.
- 16. Hirota T, Okano T, Kokame K, Shirotani-Ikejima H, Miyata T, et al. (2002) Glucose down-regulates Per1 and Per2 mRNA levels and induces circadian gene expression in cultured Rat-1 fibroblasts. J Biol Chem 277: 44244–44251.
- 17. Hochgeschwender U, Costa JL, Reed P, Bui S, Brennan MB (2003) Altered glucose homeostasis in POMC null mouse mutants lacking central and peripheral melanocortin. Endocrinology 144: 5194–5202.
- 18. Ibuka N, Inouye SI, Kawamura H (1977) Analysis of sleep-wakefulness rhythms in male rats after suprachiasmatic nucleus lesions and ocular enucleation. Brain Res 122: 33–47.
- 19. Ibuka N, Nihonmatsu I, Sekiguchi S (1980) Sleep-wakefulness rhythms in mice after suprachiasmatic nucleus lesions. Waking Sleeping 4: 167–173.
- 20. Johnson JH, Ogawa A, Chen L, Orci L, Newgard CB, et al. (1990) Underexpression of beta cell high Km glucose transporters in noninsulin-dependent diabetes. Science 250: 546–549.
- 21. King DP, Zhao Y, Sangoram AM, Wilsbacher LD, Tanaka M, et al. (1997) Positional cloning of the mouse circadian clock gene. Cell 89: 641–653.
- 22. Kita Y, Shiozawa M, Jin W, Majewski RR, Besharse JC, et al. (2002) Implications of circadian gene expression in kidney, liver and the effects of fasting on pharmacogenomic studies. Pharmacogenetics 12: 55–65.
- 23. Klaman LD, Boss O, Peroni OD, Kim JK, Martino JL, et al. (2000) Increased energy expenditure, decreased adiposity, and tissue-specific insulin sensitivity in protein-tyrosine phosphatase 1B-deficient mice. Mol Cell Biol 20: 5479–5489.
- 24. la Fleur SE, Kalsbeek A, Wortel J, Fekkes ML, Buijs RM (2001) A daily rhythm in glucose tolerance: A role for the suprachiasmatic nucleus. Diabetes 50: 1237–1243.
- 25. Linn T, Strate C, Schneider K (1999) Diet promotes beta-cell loss by apoptosis in prediabetic nonobese diabetic mice. Endocrinology 140: 3767–3773.
- 26. Maeda N, Shimomura I, Kishida K, Nishizawa H, Matsuda M, et al. (2002) Diet-induced insulin resistance in mice lacking adiponectin/ACRP30. Nat Med 8: 731–737.
- 27. Malherbe C, De Gasparo M, De Hertogh R, Hoet JJ (1969) Circadian variations of blood sugar and plasma insulin levels in man. Diabetologia 5: 397–404.
- 28. Marquez S, Crespo P, Carlini V, Garbarino-Pico E, Baler R, et al. (2004) The metabolism of phospholipids oscillates rhythmically in cultures of fibroblasts and is regulated by the clock protein PERIOD 1. FASEB J 18: 519–521.
- 29. Matsuo T, Yamaguchi S, Mitsui S, Emi A, Shimoda F, et al. (2003) Control mechanism of the circadian clock for timing of cell division in vivo. Science 302: 255–259.
- 30. McNamara P, Seo SP, Rudic RD, Sehgal A, Chakravarti D, et al. (2001) Regulation of CLOCK and MOP4 by nuclear hormone receptors in the vasculature: A humoral mechanism to reset a peripheral clock. Cell 105: 877–889.
- 31. Miller BH, Olson SL, Turek FW, Levine JE, Horton TH, et al. (2004) Circadian clock mutation disrupts estrous cyclicity and maintenance of pregnancy. Curr Biol 14: 1367–1373.
- 32. Miyake K, Ogawa W, Matsumoto M, Nakamura T, Sakaue H, et al. (2002) Hyperinsulinemia, glucose intolerance, and dyslipidemia induced by acute inhibition of phosphoinositide 3-kinase signaling in the liver. J Clin Invest 110: 1483–1491.
- 33. Mosko S, Moore RY (1979) Retinohypothalamic tract development: Alteration by suprachiasmatic lesions in the neonatal rat. Brain Res 164: 1–15.
- 34. Oakes ND, Cooney GJ, Camilleri S, Chisholm DJ, Kraegen EW (1997) Mechanisms of liver and muscle insulin resistance induced by chronic high-fat feeding. Diabetes 46: 1768–1774.
- 35. Oishi K, Miyazaki K, Kadota K, Kikuno R, Nagase T, et al. (2003) Genome-wide expression analysis of mouse liver reveals CLOCK-regulated circadian output genes. J Biol Chem 278: 41519–41527.
- 36. Pacini G, Thomaseth K, Ahren B (2001) Contribution to glucose tolerance of insulin-independent vs. insulin-dependent mechanisms in mice. Am J Physiol Endocrinol Metab 281: E693–703.
- 37. Panda S, Antoch MP, Miller BH, Su AI, Schook AB, et al. (2002) Coordinated transcription of key pathways in the mouse by the circadian clock. Cell 109: 307–320.
- 38. Reppert SM, Weaver DR (2002) Coordination of circadian timing in mammals. Nature 418: 935–941.
- 39. Schlierf G, Dorow E (1973) Diurnal patterns of triglycerides, free fatty acids, blood sugar, and insulin during carbohydrate-induction in man and their modification by nocturnal suppression of lipolysis. J Clin Invest 52: 732–740.
- 40. Seaman GV, Engel R, Swank RL, Hissen W (1965) Circadian periodicity in some physicochemical parameters of circulating blood. Nature 207: 833–835.
- 41. Stokkan KA, Yamazaki S, Tei H, Sakaki Y, Menaker M (2001) Entrainment of the circadian clock in the liver by feeding. Science 291: 490–493.
- 42. Storch KF, Lipan O, Leykin I, Viswanathan N, Davis FC, et al. (2002) Extensive and divergent circadian gene expression in liver and heart. Nature 417: 78–83.
- 43. Surwit RS, Schneider MS, Feinglos MN (1992) Stress and diabetes mellitus. Diabetes Care 15: 1413–1422.
- 44. Vitaterna MH, King DP, Chang AM, Kornhauser JM, Lowrey PL, et al. (1994) Mutagenesis and mapping of a mouse gene, Clock, essential for circadian behavior. Science 264: 719–725.
- 45. Welsh D, Richardson GS, Dement WC (1988) Effect of running wheel availability on circadian patterns of sleep and wakefulness in mice. Physiol Behav 43: 771–777.
- 46. Wimmer M (1988) A bioluminescent assay for the determination of phosphoenolpyruvate carboxykinase activity in nanogram-sized tissue samples. Anal Biochem 170: 376–381.
- 47. Yamauchi T, Kamon J, Waki H, Terauchi Y, Kubota N, et al. (2001) The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity. Nat Med 7: 941–946.
- 48. Young ME, Razeghi P, Cedars AM, Guthrie PH, Taegtmeyer H (2001) Intrinsic diurnal variations in cardiac metabolism and contractile function. Circ Res 89: 1199–1208.
- 49. Young MW, Kay SA (2001) Time zones: A comparative genetics of circadian clocks. Nat Rev Genet 2: 702–715.
- 50. Zimmet P, Alberti KG, Shaw J (2001) Global and societal implications of the diabetes epidemic. Nature 414: 782–787.