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Research Article

A Rigidifying Salt-Bridge Favors the Activity of Thermophilic Enzyme at High Temperatures at the Expense of Low-Temperature Activity

  • Sonia Y. Lam,

    Affiliation: School of Life Sciences, Centre for Protein Science and Crystallography, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China

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  • Rachel C. Y. Yeung,

    Affiliation: School of Life Sciences, Centre for Protein Science and Crystallography, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China

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  • Tsz-Ha Yu,

    Affiliation: School of Life Sciences, Centre for Protein Science and Crystallography, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China

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  • Kong-Hung Sze,

    Affiliation: Department of Chemistry, University of Hong Kong, Hong Kong SAR, China

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  • Kam-Bo Wong mail

    kbwong@cuhk.edu.hk

    Affiliation: School of Life Sciences, Centre for Protein Science and Crystallography, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China

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  • Published: March 15, 2011
  • DOI: 10.1371/journal.pbio.1001027

About the Authors

Sonia Y. Lam, Rachel C. Y. Yeung, Tsz-Ha Yu, Kam-Bo Wong
School of Life Sciences, Centre for Protein Science and Crystallography, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China
Kong-Hung Sze
Department of Chemistry, University of Hong Kong, Hong Kong SAR, China

Corresponding Author

Email: kbwong@cuhk.edu.hk

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

The author(s) have made the following declarations about their contributions: Conceived and designed the experiments: SYL KBW. Performed the experiments: SYL RCY THY. Analyzed the data: SYL RCY THY KHS KBW. Wrote the paper: SYL KBW.