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Research Article

Using Structural Information to Change the Phosphotransfer Specificity of a Two-Component Chemotaxis Signalling Complex

  • Christian H. Bell equal contributor,

    equal contributor Contributed equally to this work with: Christian H. Bell, Steven L. Porter

    Affiliations: Oxford Centre for Integrative Systems Biology, Department of Biochemistry, University of Oxford, Oxford, United Kingdom, Division of Structural Biology, University of Oxford, Oxford, United Kingdom

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  • Steven L. Porter equal contributor,

    equal contributor Contributed equally to this work with: Christian H. Bell, Steven L. Porter

    Affiliation: Oxford Centre for Integrative Systems Biology, Department of Biochemistry, University of Oxford, Oxford, United Kingdom

    Current address: School of Biosciences, University of Exeter, Exeter, United Kingdom

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  • Annabel Strawson,

    Affiliation: Oxford Centre for Integrative Systems Biology, Department of Biochemistry, University of Oxford, Oxford, United Kingdom

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  • David I. Stuart mail,

    dave@strubi.ox.ac.uk (DIS); judith.armitage@bioch.ox.ac.uk (JPA)

    Affiliation: Division of Structural Biology, University of Oxford, Oxford, United Kingdom

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  • Judith P. Armitage mail

    dave@strubi.ox.ac.uk (DIS); judith.armitage@bioch.ox.ac.uk (JPA)

    Affiliation: Oxford Centre for Integrative Systems Biology, Department of Biochemistry, University of Oxford, Oxford, United Kingdom

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  • Published: February 09, 2010
  • DOI: 10.1371/journal.pbio.1000306

About the Authors

Christian H. Bell, Steven L. Porter, Annabel Strawson, Judith P. Armitage
Oxford Centre for Integrative Systems Biology, Department of Biochemistry, University of Oxford, Oxford, United Kingdom
Christian H. Bell, David I. Stuart
Division of Structural Biology, University of Oxford, Oxford, United Kingdom

Corresponding Authors

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

The author(s) have made the following declarations about their contributions: Conceived and designed the experiments: SLP JPA. Analyzed the data: CHB SLP DIS. Wrote the paper: CHB SLP DIS JPA. Produced the constructs; crystallized the protein complexes and solved the structures; designed the CheY6 and CheY4 mutant proteins; carried out the SPR experiments: CHB. Designed the CheY6 and CheY4 mutant proteins; performed the phosphotransfer assays: SLP. Produced the constructs: AS.