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Research Article

Proteomic Studies of a Single CNS Synapse Type: The Parallel Fiber/Purkinje Cell Synapse

  • Fekrije Selimi,

    Affiliations: Laboratory of Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, New York, United States of America, CNRS, UMR7102, Paris, France, UPMC, UMR7102, Paris, France

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  • Ileana M Cristea,

    Affiliations: Laboratory for Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, New York, United States of America, Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America

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  • Elizabeth Heller,

    Affiliation: Laboratory of Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, New York, United States of America

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  • Brian T Chait,

    Affiliation: Laboratory for Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, New York, United States of America

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  • Nathaniel Heintz mail

    To whom correspondence should be addressed. E-mail: heintz@mail.rockefeller.edu

    Affiliation: Laboratory of Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, New York, United States of America

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  • Published: April 14, 2009
  • DOI: 10.1371/journal.pbio.1000083

About the Authors

Fekrije Selimi, Elizabeth Heller, Nathaniel Heintz
Laboratory of Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, New York, United States of America
Fekrije Selimi
CNRS, UMR7102, Paris, France
Fekrije Selimi
UPMC, UMR7102, Paris, France
Ileana M Cristea, Brian T Chait
Laboratory for Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, New York, United States of America
Ileana M Cristea
Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America

Corresponding Author

Email: heintz@mail.rockefeller.edu

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

FS generated the transgenic mice and performed immunofluorescence studies and functional analysis of MRCK╬│. FS and EH performed the biochemical purifications of postsynaptic densities and their western blot analysis. IMC optimized the bead conjugation protocol and performed MS analysis. FS and NH prepared the manuscript. FS, IMC, EH, BTC, and NH discussed the experiments, the data, and the manuscript.