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Research Article

Nicotinamide Riboside Kinase Structures Reveal New Pathways to NAD+

  • Wolfram Tempel equal contributor,

    equal contributor Contributed equally to this work with: Wolfram Tempel, Wael M Rabeh

    Affiliation: Structural Genomics Consortium and Department of Pharmacology, University of Toronto, Toronto, Canada

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  • Wael M Rabeh equal contributor,

    equal contributor Contributed equally to this work with: Wolfram Tempel, Wael M Rabeh

    Affiliation: Structural Genomics Consortium and Department of Pharmacology, University of Toronto, Toronto, Canada

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  • Katrina L Bogan,

    Affiliation: Departments of Genetics and Biochemistry and Norris Cotton Cancer Center, Dartmouth Medical School, Lebanon, New Hampshire, United States of America

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  • Peter Belenky,

    Affiliation: Departments of Genetics and Biochemistry and Norris Cotton Cancer Center, Dartmouth Medical School, Lebanon, New Hampshire, United States of America

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  • Marzena Wojcik,

    Affiliation: Departments of Genetics and Biochemistry and Norris Cotton Cancer Center, Dartmouth Medical School, Lebanon, New Hampshire, United States of America

    ¤a Current address: Department of Structural Biology, Chair of General Endocrinology, Medical University, Lodz, Poland

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  • Heather F Seidle,

    Affiliation: Departments of Genetics and Biochemistry and Norris Cotton Cancer Center, Dartmouth Medical School, Lebanon, New Hampshire, United States of America

    ¤b Current address: American Type Culture Collection, Manassas, Virginia, United States of America

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  • Lyudmila Nedyalkova,

    Affiliation: Structural Genomics Consortium and Department of Pharmacology, University of Toronto, Toronto, Canada

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  • Tianle Yang,

    Affiliation: Department of Pharmacology, Weill Cornell Medical College, New York, New York, United States of America

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  • Anthony A Sauve,

    Affiliation: Department of Pharmacology, Weill Cornell Medical College, New York, New York, United States of America

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  • Hee-Won Park,

    Affiliation: Structural Genomics Consortium and Department of Pharmacology, University of Toronto, Toronto, Canada

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  • Charles Brenner mail

    To whom correspondence should be addressed. E-mail: charles.brenner@dartmouth.edu

    Affiliation: Departments of Genetics and Biochemistry and Norris Cotton Cancer Center, Dartmouth Medical School, Lebanon, New Hampshire, United States of America

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  • Published: October 02, 2007
  • DOI: 10.1371/journal.pbio.0050263

About the Authors

Wolfram Tempel, Wael M Rabeh, Lyudmila Nedyalkova, Hee-Won Park
Structural Genomics Consortium and Department of Pharmacology, University of Toronto, Toronto, Canada
Katrina L Bogan, Peter Belenky, Marzena Wojcik, Heather F Seidle, Charles Brenner
Departments of Genetics and Biochemistry and Norris Cotton Cancer Center, Dartmouth Medical School, Lebanon, New Hampshire, United States of America
Tianle Yang, Anthony A Sauve
Department of Pharmacology, Weill Cornell Medical College, New York, New York, United States of America

Corresponding Author

Email: charles.brenner@dartmouth.edu

Competing Interests

AAS's institution has applied to patent the synthesis and use of NaR.

Author Contributions

CB and HWP supervised enzyme expression and purification, which was carried out by WMR, MW, HFS, and LN. WMR crystallized the enzyme. WT solved the crystal structures with HWP. PB, MW, and HFS performed mutagenesis and in vitro characterization with CB. TY performed synthesis designed by AAS. KLB demonstrated in vivo use with CB. CB coordinated the three groups and wrote the manuscript in collaboration with all contributors.