Advertisement
Research Article

High-Throughput In Vivo Analysis of Gene Expression in Caenorhabditis elegans

  • Rebecca Hunt-Newbury equal contributor,

    equal contributor Contributed equally to this work with: Rebecca Hunt-Newbury, Ryan Viveiros, Robert Johnsen

    Affiliations: Department of Zoology, University of British Columbia, Vancouver, British Columbia, Canada, Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada

    X
  • Ryan Viveiros equal contributor,

    equal contributor Contributed equally to this work with: Rebecca Hunt-Newbury, Ryan Viveiros, Robert Johnsen

    Affiliations: Department of Zoology, University of British Columbia, Vancouver, British Columbia, Canada, Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada

    X
  • Robert Johnsen equal contributor,

    equal contributor Contributed equally to this work with: Rebecca Hunt-Newbury, Ryan Viveiros, Robert Johnsen

    Affiliation: Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada

    X
  • Allan Mah,

    Affiliation: Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada

    X
  • Dina Anastas,

    Affiliations: Department of Zoology, University of British Columbia, Vancouver, British Columbia, Canada, Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada

    X
  • Lily Fang,

    Affiliation: Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada

    X
  • Erin Halfnight,

    Affiliations: Department of Zoology, University of British Columbia, Vancouver, British Columbia, Canada, Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada

    X
  • David Lee,

    Affiliation: Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada

    X
  • John Lin,

    Affiliation: Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada

    X
  • Adam Lorch,

    Affiliations: Department of Zoology, University of British Columbia, Vancouver, British Columbia, Canada, Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada

    X
  • Sheldon McKay,

    Affiliation: Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, British Columbia, Canada

    ¤ Current address: Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, United States of America

    X
  • H. Mark Okada,

    Affiliation: Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, British Columbia, Canada

    X
  • Jie Pan,

    Affiliations: Department of Zoology, University of British Columbia, Vancouver, British Columbia, Canada, Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada

    X
  • Ana K Schulz,

    Affiliation: Institut für Genetik, Technische Universität Braunschweig, Braunschweig, Germany

    X
  • Domena Tu,

    Affiliation: Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada

    X
  • Kim Wong,

    Affiliation: Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, British Columbia, Canada

    X
  • Z Zhao,

    Affiliation: Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada

    X
  • Andrey Alexeyenko,

    Affiliation: Center for Genomics and Bioinformatics, Karolinska Institutet, Stockholm, Sweden

    X
  • Thomas Burglin,

    Affiliation: Department of Biosciences, Karolinska Institutet, Huddinge, Sweden

    X
  • Eric Sonnhammer,

    Affiliation: Center for Genomics and Bioinformatics, Karolinska Institutet, Stockholm, Sweden

    X
  • Ralf Schnabel,

    Affiliation: Institut für Genetik, Technische Universität Braunschweig, Braunschweig, Germany

    X
  • Steven J Jones,

    Affiliation: Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, British Columbia, Canada

    X
  • Marco A Marra,

    Affiliation: Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, British Columbia, Canada

    X
  • David L Baillie,

    Affiliation: Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada

    X
  • Donald G Moerman mail

    To whom correspondence should be addressed. E-mail: moerman@zoology.ubc.ca

    Affiliations: Department of Zoology, University of British Columbia, Vancouver, British Columbia, Canada, Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada

    X
  • Published: September 11, 2007
  • DOI: 10.1371/journal.pbio.0050237

About the Authors

Rebecca Hunt-Newbury, Ryan Viveiros, Dina Anastas, Erin Halfnight, Adam Lorch, Jie Pan, Donald G Moerman
Department of Zoology, University of British Columbia, Vancouver, British Columbia, Canada
Rebecca Hunt-Newbury, Ryan Viveiros, Dina Anastas, Erin Halfnight, Adam Lorch, Jie Pan, Donald G Moerman
Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada
Robert Johnsen, Allan Mah, Lily Fang, David Lee, John Lin, Domena Tu, Z Zhao, David L Baillie
Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada
Sheldon McKay, H. Mark Okada, Kim Wong, Steven J Jones, Marco A Marra
Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, British Columbia, Canada
Ana K Schulz, Ralf Schnabel
Institut für Genetik, Technische Universität Braunschweig, Braunschweig, Germany
Andrey Alexeyenko, Eric Sonnhammer
Center for Genomics and Bioinformatics, Karolinska Institutet, Stockholm, Sweden
Thomas Burglin
Department of Biosciences, Karolinska Institutet, Huddinge, Sweden

Corresponding Author

Email: moerman@zoology.ubc.ca

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

DLB and DGM conceived and designed the experiments. RHN, RV, RJ, AM, DA, LF, EH, DL, JL, JP, DT, and ZZ performed the experiments. RHN, RV, AL, JP, and DGM analyzed the data. SM, HMO, AKS, KW, AA, TB, ES, RS, SJJ, MAM, and DLB contributed reagents/materials/analysis tools. RHN, RV, and DGM wrote the paper.