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Research Article

Testing Electrostatic Complementarity in Enzyme Catalysis: Hydrogen Bonding in the Ketosteroid Isomerase Oxyanion Hole

  • Daniel A Kraut,

    Affiliation: Department of Biochemistry, Stanford University, Stanford, California, United States of America

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  • Paul A Sigala,

    Affiliation: Department of Biochemistry, Stanford University, Stanford, California, United States of America

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  • Brandon Pybus,

    Affiliation: Department of Biochemistry, Brandeis University, Waltham, Massachusetts, United States of America

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  • Corey W Liu,

    Affiliation: Stanford Magnetic Resonance Laboratory, Stanford University, Stanford, California, United States of America

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  • Dagmar Ringe,

    Affiliation: Department of Biochemistry, Brandeis University, Waltham, Massachusetts, United States of America

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  • Gregory A Petsko,

    Affiliation: Department of Biochemistry, Brandeis University, Waltham, Massachusetts, United States of America

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  • Daniel Herschlag mail

    To whom correspondence should be addressed. E-mail: herschla@stanford.edu

    Affiliation: Department of Biochemistry, Stanford University, Stanford, California, United States of America

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  • Published: March 28, 2006
  • DOI: 10.1371/journal.pbio.0040099

About the Authors

Daniel A Kraut, Paul A Sigala, Daniel Herschlag
Department of Biochemistry, Stanford University, Stanford, California, United States of America
Brandon Pybus, Dagmar Ringe, Gregory A Petsko
Department of Biochemistry, Brandeis University, Waltham, Massachusetts, United States of America
Corey W Liu
Stanford Magnetic Resonance Laboratory, Stanford University, Stanford, California, United States of America

Corresponding Author

Email: herschla@stanford.edu

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

DAK, PAS, and DH conceived and designed the experiments, BP performed X-ray crystallography experiments, PAS performed 1D 1H and 19F NMR experiments, PAS and CWL performed 2D NOESY NMR experiments, DAK carried out assay development and performed binding assays and ITC experiments. DAK, PAS, BP, CWL, DR, GAP, and DH analyzed the data. DAK, PAS, BP, CWL, DR, GAP, and DH wrote the paper.