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Research Article

The Genome Sequence of Rickettsia felis Identifies the First Putative Conjugative Plasmid in an Obligate Intracellular Parasite

  • Hiroyuki Ogata mail,

    To whom correspondence should be addressed. E-mail: Hiroyuki.Ogata@igs.cnrs-mrs.fr (HO), Email: Didier.Raoult@medecine.univ-mrs.fr (DR)

    Affiliation: Structural and Genomic Information Laboratory, UPR 2589, IBSM, CNRS, Marseille Cedex, France

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  • Patricia Renesto,

    Affiliation: Unité des Rickettsies, UMR 6020, IFR 48, CNRS, Faculté de Médecine, Marseille Cedex, France

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  • Stéphane Audic,

    Affiliation: Structural and Genomic Information Laboratory, UPR 2589, IBSM, CNRS, Marseille Cedex, France

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  • Catherine Robert,

    Affiliation: Unité des Rickettsies, UMR 6020, IFR 48, CNRS, Faculté de Médecine, Marseille Cedex, France

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  • Guillaume Blanc,

    Affiliation: Structural and Genomic Information Laboratory, UPR 2589, IBSM, CNRS, Marseille Cedex, France

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  • Pierre-Edouard Fournier,

    Affiliations: Structural and Genomic Information Laboratory, UPR 2589, IBSM, CNRS, Marseille Cedex, France, Unité des Rickettsies, UMR 6020, IFR 48, CNRS, Faculté de Médecine, Marseille Cedex, France

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  • Hugues Parinello,

    Affiliation: Unité des Rickettsies, UMR 6020, IFR 48, CNRS, Faculté de Médecine, Marseille Cedex, France

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  • Jean-Michel Claverie,

    Affiliation: Structural and Genomic Information Laboratory, UPR 2589, IBSM, CNRS, Marseille Cedex, France

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  • Didier Raoult mail

    To whom correspondence should be addressed. E-mail: Hiroyuki.Ogata@igs.cnrs-mrs.fr (HO), Email: Didier.Raoult@medecine.univ-mrs.fr (DR)

    Affiliation: Unité des Rickettsies, UMR 6020, IFR 48, CNRS, Faculté de Médecine, Marseille Cedex, France

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  • Published: July 05, 2005
  • DOI: 10.1371/journal.pbio.0030248

About the Authors

Hiroyuki Ogata, Stéphane Audic, Guillaume Blanc, Pierre-Edouard Fournier, Jean-Michel Claverie
Structural and Genomic Information Laboratory, UPR 2589, IBSM, CNRS, Marseille Cedex, France
Patricia Renesto, Catherine Robert, Pierre-Edouard Fournier, Hugues Parinello, Didier Raoult
Unité des Rickettsies, UMR 6020, IFR 48, CNRS, Faculté de Médecine, Marseille Cedex, France

Corresponding Authors

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

HO performed genome annotation and coordinated the bioinformatics analyses. PR performed electron microscopic analysis of pili, immunofluorescence assay of actin-based motility, and hemolytic activity assay. SA and CR assembled genome sequences, performed sequence finishing, and carried out experiments to characterize the plasmids. CR constructed genomic libraries. GB performed most phylogenetic analyses. PEF performed β-lactamase activity assay and contributed to the bioinformatics analyses. HP contributed to the sequencing. JMC provided laboratory (computing) support and supplied ideas. DR provided laboratory (experimental) support, supplied ideas, and coordinated the experimental aspects of the work. All authors contributed in drafting the manuscript.