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Research Article

Site-Directed Spin Labeling Reveals Pentameric Ligand-Gated Ion Channel Gating Motions

  • Cosma D. Dellisanti,

    Affiliation: Department of Neuroscience, University of Wisconsin, Madison, Wisconsin, United States of America

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  • Borna Ghosh,

    Affiliation: Department of Neuroscience, University of Wisconsin, Madison, Wisconsin, United States of America

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  • Susan M. Hanson,

    Affiliation: Department of Neuroscience, University of Wisconsin, Madison, Wisconsin, United States of America

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  • James M. Raspanti,

    Affiliation: Department of Neuroscience, University of Wisconsin, Madison, Wisconsin, United States of America

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  • Valerie A. Grant,

    Affiliation: Department of Neuroscience, University of Wisconsin, Madison, Wisconsin, United States of America

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  • Gaoussou M. Diarra,

    Affiliation: Department of Neuroscience, University of Wisconsin, Madison, Wisconsin, United States of America

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  • Abby M. Schuh,

    Affiliation: Department of Neuroscience, University of Wisconsin, Madison, Wisconsin, United States of America

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  • Kenneth Satyshur,

    Affiliation: Department of Neuroscience, University of Wisconsin, Madison, Wisconsin, United States of America

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  • Candice S. Klug,

    Affiliation: Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America

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  • Cynthia Czajkowski mail

    cmczajko@wisc.edu

    Affiliation: Department of Neuroscience, University of Wisconsin, Madison, Wisconsin, United States of America

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  • Published: November 19, 2013
  • DOI: 10.1371/journal.pbio.1001714

About the Authors

Cosma D. Dellisanti, Borna Ghosh, Susan M. Hanson, James M. Raspanti, Valerie A. Grant, Gaoussou M. Diarra, Abby M. Schuh, Kenneth Satyshur, Cynthia Czajkowski
Department of Neuroscience, University of Wisconsin, Madison, Wisconsin, United States of America
Candice S. Klug
Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America

Corresponding Author

Email: cmczajko@wisc.edu

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

The author(s) have made the following declarations about their contributions: Conceived and designed the experiments: CDD SMH CSK CC. Performed the experiments: CDD BG SMH JMR VAG GMD AMS KS CSK. Analyzed and discussed the data: CDD CSK CC. Contributed reagents/materials/analysis tools: CSK CC. Wrote and edited the paper: CDD CSK CC. Made mutants: CDD VAG SMH BG. Conducted two-electrode voltage clamp recordings in oocytes: SMH BG. Purified the protein: CDD JMR VAG GMD AMS. Reconstituted GLIC protein into liposomes, recorded and analyzed single-channel currents in planar lipid bilayers: CDD. Collected and analyzed EPR data: CSK. Built homology model of GLIC: BG KS. Used computer modeling to estimate interspin distances: KS. Supervised the project: CC.