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Research Article

Anthranilate Fluorescence Marks a Calcium-Propagated Necrotic Wave That Promotes Organismal Death in C. elegans

  • Cassandra Coburn,

    Affiliation: Institute of Healthy Ageing, and Research Department of Genetics, Evolution and Environment, University College London, London, United Kingdom

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  • Erik Allman,

    Affiliation: Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, New York, United States of America

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  • Parag Mahanti,

    Affiliation: Boyce Thompson Institute and Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York, United States of America

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  • Alexandre Benedetto,

    Affiliation: Institute of Healthy Ageing, and Research Department of Genetics, Evolution and Environment, University College London, London, United Kingdom

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  • Filipe Cabreiro,

    Affiliation: Institute of Healthy Ageing, and Research Department of Genetics, Evolution and Environment, University College London, London, United Kingdom

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  • Zachary Pincus,

    Affiliation: Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut, United States of America

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  • Filip Matthijssens,

    Affiliation: Department of Biology, Ghent University, Ghent, Belgium

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  • Caroline Araiz,

    Affiliation: Institute of Healthy Ageing, and Research Department of Genetics, Evolution and Environment, University College London, London, United Kingdom

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  • Abraham Mandel,

    Affiliation: Institute of Healthy Ageing, and Research Department of Genetics, Evolution and Environment, University College London, London, United Kingdom

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  • Manolis Vlachos,

    Affiliation: Institute of Molecular Biology and Biotechnology, University of Crete, Heraklion, Crete, Greece

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  • Sally-Anne Edwards,

    Affiliation: Institute of Healthy Ageing, and Research Department of Genetics, Evolution and Environment, University College London, London, United Kingdom

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  • Grahame Fischer,

    Affiliation: Institute of Healthy Ageing, and Research Department of Genetics, Evolution and Environment, University College London, London, United Kingdom

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  • Alexander Davidson,

    Affiliation: Institute of Healthy Ageing, and Research Department of Genetics, Evolution and Environment, University College London, London, United Kingdom

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  • Rosina E. Pryor,

    Affiliation: Institute of Healthy Ageing, and Research Department of Genetics, Evolution and Environment, University College London, London, United Kingdom

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  • Ailsa Stevens,

    Affiliation: Institute of Healthy Ageing, and Research Department of Genetics, Evolution and Environment, University College London, London, United Kingdom

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  • Frank J. Slack,

    Affiliation: Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut, United States of America

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  • Nektarios Tavernarakis,

    Affiliation: Institute of Molecular Biology and Biotechnology, University of Crete, Heraklion, Crete, Greece

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  • Bart P. Braeckman,

    Affiliation: Department of Biology, Ghent University, Ghent, Belgium

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  • Frank C. Schroeder,

    Affiliation: Boyce Thompson Institute and Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York, United States of America

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  • Keith Nehrke,

    Affiliations: Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, New York, United States of America, Department of Medicine, University of Rochester Medical Center, Rochester, New York, United States of America

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  • David Gems mail

    david.gems@ucl.ac.uk

    Affiliation: Institute of Healthy Ageing, and Research Department of Genetics, Evolution and Environment, University College London, London, United Kingdom

    X
  • Published: July 23, 2013
  • DOI: 10.1371/journal.pbio.1001613

About the Authors

Cassandra Coburn, Alexandre Benedetto, Filipe Cabreiro, Caroline Araiz, Abraham Mandel, Sally-Anne Edwards, Grahame Fischer, Alexander Davidson, Rosina E. Pryor, Ailsa Stevens, David Gems
Institute of Healthy Ageing, and Research Department of Genetics, Evolution and Environment, University College London, London, United Kingdom
Erik Allman, Keith Nehrke
Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, New York, United States of America
Parag Mahanti, Frank C. Schroeder
Boyce Thompson Institute and Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York, United States of America
Zachary Pincus, Frank J. Slack
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut, United States of America
Filip Matthijssens, Bart P. Braeckman
Department of Biology, Ghent University, Ghent, Belgium
Manolis Vlachos, Nektarios Tavernarakis
Institute of Molecular Biology and Biotechnology, University of Crete, Heraklion, Crete, Greece
Keith Nehrke
Department of Medicine, University of Rochester Medical Center, Rochester, New York, United States of America

Corresponding Author

Email: david.gems@ucl.ac.uk

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

The author(s) have made the following declarations about their contributions: Conceived and designed the experiments: CC DG KN PM AB FC. Performed the experiments: CC AD SAE AS FS ZP PM BB FM AM GF EA KN MV NT AB CA FC REP. Analyzed the data: CC DG KN EA PM FS BB FM MV NT AB. Contributed reagents/materials/analysis tools: PM FS EA FJS KN FM BB. Wrote the paper: CC DG KN. AS worked as an undergraduate project student to identify the severity of different Glo mutant phenotypes, consequently allowing us to select glo-1(zu347) as the primary mutant of study.