Advertisement
Research Article

A Genetically Encoded Tag for Correlated Light and Electron Microscopy of Intact Cells, Tissues, and Organisms

  • Xiaokun Shu mail,

    rtsien@ucsd.edu (RYT); xiaokun.shu@ucsf.edu (XS)

    Affiliations: Howard Hughes Medical Institute, University of California at San Diego, La Jolla, California, United States of America, Department of Pharmacology, University of California at San Diego, La Jolla, California, United States of America

    Current address: Department of Pharmaceutical Chemistry and Cardiovascular Research Institute, University of California at San Francisco, San Francisco, California, United States of America

    X
  • Varda Lev-Ram,

    Affiliation: Department of Pharmacology, University of California at San Diego, La Jolla, California, United States of America

    X
  • Thomas J. Deerinck,

    Affiliation: National Center for Microscopy and Imaging Research, Center for Research on Biological Systems, University of California at San Diego, La Jolla, California, United States of America

    X
  • Yingchuan Qi,

    Affiliations: Howard Hughes Medical Institute, University of California at San Diego, La Jolla, California, United States of America, Division of Biological Science, Section of Neurobiology, University of California at San Diego, La Jolla, California, United States of America

    X
  • Ericka B. Ramko,

    Affiliation: National High Magnetic Field Laboratory and Department of Biological Science, The Florida State University, Tallahassee, Florida, United States of America

    X
  • Michael W. Davidson,

    Affiliation: National High Magnetic Field Laboratory and Department of Biological Science, The Florida State University, Tallahassee, Florida, United States of America

    X
  • Yishi Jin,

    Affiliations: Howard Hughes Medical Institute, University of California at San Diego, La Jolla, California, United States of America, Division of Biological Science, Section of Neurobiology, University of California at San Diego, La Jolla, California, United States of America

    X
  • Mark H. Ellisman,

    Affiliations: National Center for Microscopy and Imaging Research, Center for Research on Biological Systems, University of California at San Diego, La Jolla, California, United States of America, Department of Neurosciences, University of California at San Diego, La Jolla, California, United States of America,

    X
  • Roger Y. Tsien mail

    rtsien@ucsd.edu (RYT); xiaokun.shu@ucsf.edu (XS)

    Affiliations: Howard Hughes Medical Institute, University of California at San Diego, La Jolla, California, United States of America, Department of Pharmacology, University of California at San Diego, La Jolla, California, United States of America, Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, California, United States of America

    X
  • Published: April 05, 2011
  • DOI: 10.1371/journal.pbio.1001041

About the Authors

Xiaokun Shu, Yingchuan Qi, Yishi Jin, Roger Y. Tsien
Howard Hughes Medical Institute, University of California at San Diego, La Jolla, California, United States of America
Xiaokun Shu, Varda Lev-Ram, Roger Y. Tsien
Department of Pharmacology, University of California at San Diego, La Jolla, California, United States of America
Thomas J. Deerinck, Mark H. Ellisman
National Center for Microscopy and Imaging Research, Center for Research on Biological Systems, University of California at San Diego, La Jolla, California, United States of America
Yingchuan Qi, Yishi Jin
Division of Biological Science, Section of Neurobiology, University of California at San Diego, La Jolla, California, United States of America
Ericka B. Ramko, Michael W. Davidson
National High Magnetic Field Laboratory and Department of Biological Science, The Florida State University, Tallahassee, Florida, United States of America
Mark H. Ellisman
Department of Neurosciences, University of California at San Diego, La Jolla, California, United States of America,
Roger Y. Tsien
Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, California, United States of America

Corresponding Authors

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

The author(s) have made the following declarations about their contributions: Conceived and designed the experiments: XS RYT. Performed the experiments: XS VL TJD YQ EBR MWD. Analyzed the data: XS VL TJD YQ MWD YJ MHE RYT. Contributed reagents/materials/analysis tools: XS VL TJD YQ EBR MWD YJ MHE RYT. Wrote the paper: XS MHE RYT. Proposal of engineering FMN-binding phototropins as genetically encoded and efficient SOGs, with following research design, engineering, and characterization of miniSOG: XS. Coordination and guidance of correlated LM/EM: MHE. Coordination and proposal of the desirability of a genetically encoded SOG: RYT.