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Research Article

Kissing G Domains of MnmE Monitored by X-Ray Crystallography and Pulse Electron Paramagnetic Resonance Spectroscopy

  • Simon Meyer,

    Affiliation: Department of Structural Biology, Max-Planck-Institute of Molecular Physiology, Dortmund, Germany

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  • Sabine Böhme,

    Affiliation: Department of Physics, University of Osnabrück, Osnabrück, Germany

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  • André Krüger,

    Affiliation: Department of Structural Biology, Max-Planck-Institute of Molecular Physiology, Dortmund, Germany

    Current address: Center for Cellular Imaging and Nanoanalytics, M.E. Mueller Institute for Structural Biology, Biozentrum, University of Basel, Basel, Switzerland

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  • Heinz-Jürgen Steinhoff,

    Affiliation: Department of Physics, University of Osnabrück, Osnabrück, Germany

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  • Johann P. Klare mail,

    jklare@uos.de (JPK); alfred.wittinghofer@mpi-dortmund.mpg.de (AW)

    Affiliation: Department of Physics, University of Osnabrück, Osnabrück, Germany

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  • Alfred Wittinghofer mail

    jklare@uos.de (JPK); alfred.wittinghofer@mpi-dortmund.mpg.de (AW)

    Affiliation: Department of Structural Biology, Max-Planck-Institute of Molecular Physiology, Dortmund, Germany

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  • Published: October 06, 2009
  • DOI: 10.1371/journal.pbio.1000212

About the Authors

Simon Meyer, André Krüger, Alfred Wittinghofer
Department of Structural Biology, Max-Planck-Institute of Molecular Physiology, Dortmund, Germany
Sabine Böhme, Heinz-Jürgen Steinhoff, Johann P. Klare
Department of Physics, University of Osnabrück, Osnabrück, Germany

Corresponding Authors

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

The author(s) have made the following declarations about their contributions: Conceived and designed the experiments: JPK AW. Performed the experiments: SM SB AK JPK. Analyzed the data: SM SB JPK AW. Contributed reagents/materials/analysis tools: HJS. Wrote the paper: SM SB HJS JPK AW. Performed the protein preparations and biochemical experiments, performed the crystallization experiments, X-ray data analysis, and solving of the X-ray structures: SM. Performed and analyzed the EPR experiments: SB JPK. Designed the mutants: SM AK JPK. Provided the EPR equipment: H-JS. Performed the protein preparations and biochemical experiments: AK.