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Research Article

The Active Site of a Carbohydrate Esterase Displays Divergent Catalytic and Noncatalytic Binding Functions

  • Cedric Montanier equal contributor,

    equal contributor Contributed equally to this work with: Cedric Montanier, Victoria A Money

    Affiliation: Institute for Cell and Molecular Biosciences, Newcastle University, The Medical School, Newcastle upon Tyne, United Kingdom

    X
  • Victoria A Money equal contributor,

    equal contributor Contributed equally to this work with: Cedric Montanier, Victoria A Money

    Affiliation: York Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York, United Kingdom

    X
  • Virginia M. R Pires,

    Affiliation: CIISA - Faculdade de Medicina Veterinária, Universidade Técnica de Lisboa, Avenida da Universidade Técnica, Lisboa, Portugal

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  • James E Flint,

    Affiliation: Institute for Cell and Molecular Biosciences, Newcastle University, The Medical School, Newcastle upon Tyne, United Kingdom

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  • Benedita A Pinheiro,

    Affiliation: CIISA - Faculdade de Medicina Veterinária, Universidade Técnica de Lisboa, Avenida da Universidade Técnica, Lisboa, Portugal

    X
  • Arun Goyal,

    Affiliation: CIISA - Faculdade de Medicina Veterinária, Universidade Técnica de Lisboa, Avenida da Universidade Técnica, Lisboa, Portugal

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  • José A. M Prates,

    Affiliation: CIISA - Faculdade de Medicina Veterinária, Universidade Técnica de Lisboa, Avenida da Universidade Técnica, Lisboa, Portugal

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  • Atsushi Izumi,

    Affiliation: York Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York, United Kingdom

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  • Henrik Stålbrand,

    Affiliation: Department of Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, Lund, Sweden

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  • Carl Morland,

    Affiliation: Institute for Cell and Molecular Biosciences, Newcastle University, The Medical School, Newcastle upon Tyne, United Kingdom

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  • Alan Cartmell,

    Affiliation: Institute for Cell and Molecular Biosciences, Newcastle University, The Medical School, Newcastle upon Tyne, United Kingdom

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  • Katarina Kolenova,

    Affiliation: Department of Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, Lund, Sweden

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  • Evangelos Topakas,

    Affiliation: Institute for Cell and Molecular Biosciences, Newcastle University, The Medical School, Newcastle upon Tyne, United Kingdom

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  • Eleanor J Dodson,

    Affiliation: York Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York, United Kingdom

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  • David N Bolam,

    Affiliation: Institute for Cell and Molecular Biosciences, Newcastle University, The Medical School, Newcastle upon Tyne, United Kingdom

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  • Gideon J Davies mail,

    To whom correspondence should be addressed. E-mail: davies@ysbl.york.ac.uk (GJD); H.J.Gilbert@ncl.ac.uk (HJG)

    Affiliation: York Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York, United Kingdom

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  • Carlos M. G. A Fontes,

    Affiliation: CIISA - Faculdade de Medicina Veterinária, Universidade Técnica de Lisboa, Avenida da Universidade Técnica, Lisboa, Portugal

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  • Harry J Gilbert mail

    To whom correspondence should be addressed. E-mail: davies@ysbl.york.ac.uk (GJD); H.J.Gilbert@ncl.ac.uk (HJG)

    Affiliation: Institute for Cell and Molecular Biosciences, Newcastle University, The Medical School, Newcastle upon Tyne, United Kingdom

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  • Published: March 31, 2009
  • DOI: 10.1371/journal.pbio.1000071

About the Authors

Cedric Montanier, James E Flint, Carl Morland, Alan Cartmell, Evangelos Topakas, David N Bolam, Harry J Gilbert
Institute for Cell and Molecular Biosciences, Newcastle University, The Medical School, Newcastle upon Tyne, United Kingdom
Victoria A Money, Atsushi Izumi, Eleanor J Dodson, Gideon J Davies
York Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York, United Kingdom
Virginia M. R Pires, Benedita A Pinheiro, Arun Goyal, José A. M Prates, Carlos M. G. A Fontes
CIISA - Faculdade de Medicina Veterinária, Universidade Técnica de Lisboa, Avenida da Universidade Técnica, Lisboa, Portugal
Henrik Stålbrand, Katarina Kolenova
Department of Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, Lund, Sweden

Corresponding Authors

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

CM, JEF, HS, AC, ET, DNB, GJD, CMGAF, and HJG conceived and designed the experiments. CM, VAM, VMRP, JEF, BAP, AG, JAMP, AI, HS, CM, AC, ET, DNB, GJD, and CMGAF performed the experiments. CM, VAM, VMRP, JEF, BAP, AG, JAMP, AI, HS, CM, AC, KK, ET, EJD, DNB, GJD, CMGAF, and HJG analyzed the data. KK contributed reagents/materials/analysis tools. DNB, GJD, and HJG wrote the paper.