Advertisement
Research Article

A Microtubule Interactome: Complexes with Roles in Cell Cycle and Mitosis

  • Julian R Hughes equal contributor,

    equal contributor Contributed equally to this work with: Julian R Hughes, Ana M Meireles, Katherine H Fisher

    Affiliation: Department of Zoology, University of Oxford, Oxford, United Kingdom

    ¤a Current address: Sareum Ltd., Cambridge, United Kingdom

    X
  • Ana M Meireles equal contributor,

    equal contributor Contributed equally to this work with: Julian R Hughes, Ana M Meireles, Katherine H Fisher

    Affiliations: Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, United Kingdom, Programa Doutoral em Biologia Experimental e Biomedicina, Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal

    X
  • Katherine H Fisher equal contributor,

    equal contributor Contributed equally to this work with: Julian R Hughes, Ana M Meireles, Katherine H Fisher

    Affiliations: Department of Zoology, University of Oxford, Oxford, United Kingdom, Life Sciences Interface/Doctoral Training Centre, University of Oxford, Oxford, United Kingdom, Department of Biochemistry, University of Oxford, Oxford, United Kingdom

    X
  • Angel Garcia,

    Affiliation: Department of Statistics, University of Oxford, Oxford, United Kingdom

    ¤b Current address: Universidade de Santiago de Compostela, Santiago de Compostela, Spain

    X
  • Philip R Antrobus,

    Affiliation: Department of Statistics, University of Oxford, Oxford, United Kingdom

    X
  • Alan Wainman,

    Affiliation: Department of Zoology, University of Oxford, Oxford, United Kingdom

    X
  • Nicole Zitzmann,

    Affiliation: Department of Statistics, University of Oxford, Oxford, United Kingdom

    X
  • Charlotte Deane,

    Affiliation: Department of Biochemistry, University of Oxford, Oxford, United Kingdom

    X
  • Hiroyuki Ohkura,

    Affiliation: Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, United Kingdom

    X
  • James G Wakefield mail

    To whom correspondence should be addressed. E-mail: james.wakefield@zoo.ox.ac.uk

    Affiliations: Department of Zoology, University of Oxford, Oxford, United Kingdom, Life Sciences Interface/Doctoral Training Centre, University of Oxford, Oxford, United Kingdom

    X
  • Published: April 22, 2008
  • DOI: 10.1371/journal.pbio.0060098

About the Authors

Julian R Hughes, Katherine H Fisher, Alan Wainman, James G Wakefield
Department of Zoology, University of Oxford, Oxford, United Kingdom
Ana M Meireles, Hiroyuki Ohkura
Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, United Kingdom
Ana M Meireles
Programa Doutoral em Biologia Experimental e Biomedicina, Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal
Katherine H Fisher, James G Wakefield
Life Sciences Interface/Doctoral Training Centre, University of Oxford, Oxford, United Kingdom
Angel Garcia, Philip R Antrobus, Nicole Zitzmann
Department of Statistics, University of Oxford, Oxford, United Kingdom
Katherine H Fisher, Charlotte Deane
Department of Biochemistry, University of Oxford, Oxford, United Kingdom

Corresponding Author

Email: james.wakefield@zoo.ox.ac.uk

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

The MT cosedimentation assays were undertaken by JRH and JGW. Proteomic analyses were carried out by AG and PRA, and supervised by NZ. All RNAi experiments were undertaken by AMM and supervised by HO. Bioinformatics analyses were undertaken by KHF and supervised by CD. Biochemical analyses of complexes were carried out by JRH and KHF. Immunolocalisation of complexes and the localisation of GFP-SkAP were undertaken by AW. The overall experimental rationale was designed by JRH and JGW.