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Research Article

Intramolecular and Intermolecular Interactions of Protein Kinase B Define Its Activation In Vivo

  • Véronique Calleja equal contributor,

    equal contributor Contributed equally to this work with: Véronique Calleja, Damien Alcor

    Affiliation: Cell Biophysics Laboratory, Lincoln's Inn Fields Laboratories, London Research Institute, Cancer Research UK, London, United Kingdom

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  • Damien Alcor equal contributor,

    equal contributor Contributed equally to this work with: Véronique Calleja, Damien Alcor

    Affiliation: Cell Biophysics Laboratory, Lincoln's Inn Fields Laboratories, London Research Institute, Cancer Research UK, London, United Kingdom

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  • Michel Laguerre,

    Affiliation: Institut Européen de Chimie et Biologie, Pessac Cedex, France

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  • Jongsun Park,

    Affiliation: Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland

    ¤ Current address: Cell Signaling Laboratory, Cancer Research Institute, College of Medicine, Chungnam National University, Taejon, South Korea

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  • Borivoj Vojnovic,

    Affiliation: Advanced Technology Development Group, Gray Cancer Institute, Mount Vernon Hospital, Northwood, United Kingdom

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  • Brian A Hemmings,

    Affiliation: Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland

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  • Julian Downward,

    Affiliation: Signal Transduction Laboratory, Lincoln's Inn Fields Laboratories, London Research Institute, Cancer Research UK, London, United Kingdom

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  • Peter J Parker mail,

    To whom correspondence should be addressed. E-mail: peter.parker@cancer.org.uk (PJP); banafshe.larijani@cancer.org.uk (BL)

    Affiliation: Protein Phosphorylation Laboratory, Lincoln's Inn Fields Laboratories, London Research Institute, Cancer Research UK, London, United Kingdom

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  • Banafshé Larijani mail

    To whom correspondence should be addressed. E-mail: peter.parker@cancer.org.uk (PJP); banafshe.larijani@cancer.org.uk (BL)

    Affiliation: Cell Biophysics Laboratory, Lincoln's Inn Fields Laboratories, London Research Institute, Cancer Research UK, London, United Kingdom

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  • Published: April 03, 2007
  • DOI: 10.1371/journal.pbio.0050095

About the Authors

Véronique Calleja, Damien Alcor, Banafshé Larijani
Cell Biophysics Laboratory, Lincoln's Inn Fields Laboratories, London Research Institute, Cancer Research UK, London, United Kingdom
Michel Laguerre
Institut Européen de Chimie et Biologie, Pessac Cedex, France
Jongsun Park, Brian A Hemmings
Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
Borivoj Vojnovic
Advanced Technology Development Group, Gray Cancer Institute, Mount Vernon Hospital, Northwood, United Kingdom
Julian Downward
Signal Transduction Laboratory, Lincoln's Inn Fields Laboratories, London Research Institute, Cancer Research UK, London, United Kingdom
Peter J Parker
Protein Phosphorylation Laboratory, Lincoln's Inn Fields Laboratories, London Research Institute, Cancer Research UK, London, United Kingdom

Corresponding Authors

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

VC, DA, and BL were involved in the design and performed the FRET experiments. VC was responsible for the design of the molecular biology tools and performed the biochemical experiments. ML was responsible for the molecular modeling. JP, BAH, and JD provided DNA constructs. VC, DA, BV, and BL were responsible for the set-up and optimisation of two-photon FLIM. BAH and JD were involved in scientific discussions. PJP and BL supervised the project. VC, DA, PJP, and BL were involved in writing the manuscript.