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Research Article

A Genetically Encoded Tag for Correlated Light and Electron Microscopy of Intact Cells, Tissues, and Organisms

  • Xiaokun Shu mail,

    rtsien@ucsd.edu (RYT); xiaokun.shu@ucsf.edu (XS)

    Affiliations: Howard Hughes Medical Institute, University of California at San Diego, La Jolla, California, United States of America, Department of Pharmacology, University of California at San Diego, La Jolla, California, United States of America

    Current address: Department of Pharmaceutical Chemistry and Cardiovascular Research Institute, University of California at San Francisco, San Francisco, California, United States of America

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  • Varda Lev-Ram,

    Affiliation: Department of Pharmacology, University of California at San Diego, La Jolla, California, United States of America

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  • Thomas J. Deerinck,

    Affiliation: National Center for Microscopy and Imaging Research, Center for Research on Biological Systems, University of California at San Diego, La Jolla, California, United States of America

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  • Yingchuan Qi,

    Affiliations: Howard Hughes Medical Institute, University of California at San Diego, La Jolla, California, United States of America, Division of Biological Science, Section of Neurobiology, University of California at San Diego, La Jolla, California, United States of America

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  • Ericka B. Ramko,

    Affiliation: National High Magnetic Field Laboratory and Department of Biological Science, The Florida State University, Tallahassee, Florida, United States of America

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  • Michael W. Davidson,

    Affiliation: National High Magnetic Field Laboratory and Department of Biological Science, The Florida State University, Tallahassee, Florida, United States of America

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  • Yishi Jin,

    Affiliations: Howard Hughes Medical Institute, University of California at San Diego, La Jolla, California, United States of America, Division of Biological Science, Section of Neurobiology, University of California at San Diego, La Jolla, California, United States of America

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  • Mark H. Ellisman,

    Affiliations: National Center for Microscopy and Imaging Research, Center for Research on Biological Systems, University of California at San Diego, La Jolla, California, United States of America, Department of Neurosciences, University of California at San Diego, La Jolla, California, United States of America,

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  • Roger Y. Tsien mail

    rtsien@ucsd.edu (RYT); xiaokun.shu@ucsf.edu (XS)

    Affiliations: Howard Hughes Medical Institute, University of California at San Diego, La Jolla, California, United States of America, Department of Pharmacology, University of California at San Diego, La Jolla, California, United States of America, Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, California, United States of America

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  • Published: April 05, 2011
  • DOI: 10.1371/journal.pbio.1001041

Reader Comments (3)

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Utility of miniSOG compared to other photoreceptor-derived tools

Posted by cfaulkner on 26 May 2011 at 08:34 GMT

This article has developed a new tool with exciting applications relating to both light and electron microscopy. Readers and potential users may be interested to compare the utility of miniSOG with iLOV (Chapman et al., 2008, PNAS 105:20038; doi: 10.1073/pnas0807551105) and FbFPs (Drepper et al., 2007, Nature Biotech. 25:443; doi: 10.1038/nbt1293) which are tools originating from the same or similar starting sequences.

Competing interests declared: I am an author on Chapman et al. (2008)