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Research Article

Mechanistic Insights Revealed by the Crystal Structure of a Histidine Kinase with Signal Transducer and Sensor Domains

  • Chen Wang equal contributor,

    equal contributor Contributed equally to this work with: Chen Wang, Jiayan Sang

    Affiliations: State Key Laboratory for Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiangan, Xiamen, China, Department of Molecular and Computational Biology, University of Southern California, Los Angeles, California, United States of America

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  • Jiayan Sang equal contributor,

    equal contributor Contributed equally to this work with: Chen Wang, Jiayan Sang

    Affiliation: State Key Laboratory for Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiangan, Xiamen, China

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  • Jiawei Wang,

    Affiliation: Department of Biology and Technology, Tsinghua University, Beijing, China

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  • Mingyan Su,

    Affiliation: State Key Laboratory for Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiangan, Xiamen, China

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  • Jennifer S. Downey,

    Affiliation: Division of Biomedical Science, Herman Ostrow School of Dentistry of University of Southern California, Los Angeles, California, United States of America

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  • Qinggan Wu,

    Affiliation: State Key Laboratory for Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiangan, Xiamen, China

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  • Shida Wang,

    Affiliation: Dental Research Institute, Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada

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  • Yongfei Cai,

    Affiliation: State Key Laboratory for Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiangan, Xiamen, China

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  • Xiaozheng Xu,

    Affiliation: State Key Laboratory for Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiangan, Xiamen, China

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  • Jun Wu,

    Affiliation: State Key Laboratory for Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiangan, Xiamen, China

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  • Dilani B. Senadheera,

    Affiliation: Dental Research Institute, Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada

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  • Dennis G. Cvitkovitch,

    Affiliation: Dental Research Institute, Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada

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  • Lin Chen,

    Affiliation: Department of Molecular and Computational Biology, University of Southern California, Los Angeles, California, United States of America

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  • Steven D. Goodman,

    Affiliation: Division of Biomedical Science, Herman Ostrow School of Dentistry of University of Southern California, Los Angeles, California, United States of America

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  • Aidong Han mail

    ahan@xmu.edu.cn

    Affiliation: State Key Laboratory for Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiangan, Xiamen, China

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  • Published: February 26, 2013
  • DOI: 10.1371/journal.pbio.1001493

Reader Comments (4)

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PLOS Biologue blog post

Posted by RoliRoberts on 28 Feb 2013 at 18:01 GMT

See the blog post on PLOS Biologue: http://blogs.plos.org/bio...

No competing interests declared.

RE: PLOS Biologue blog post

ahan replied to RoliRoberts on 08 Mar 2013 at 01:56 GMT

Dr. Roberts has created a great picture by putting together ours with the work from Dr. Crane and his colleague. I strongly suggest he write another comprehensive comment/review about this idea for a formal publication. The two things I like to point out here. One is that VicK HAMP has unique packing (Figure 2), which may be more stable than other homologues. How the HAMP changes its conformation is still puzzling to us. The second is that VicK PAS domain is not found to bind by any ligands. This is a major drawback of VicK used in studying molecular mechanisms of signal transduction. Therefore, we have not been able to analyze any upstream conformational changes in context with downstream DHp domain bending and CA positioning in this paper. We are currently performing molecular dynamics study, hoping to find some insights of conformational transitions.

No competing interests declared.