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Research Article

The Hierarchy of Exon-Junction Complex Assembly by the Spliceosome Explains Key Features of Mammalian Nonsense-Mediated mRNA Decay

  • Niels H. Gehring mail,

    Niels.Gehring@med.uni-heidelberg.de (NHG); Andreas.Kulozik@med.uni-heidelberg.de (AEK)

    Affiliations: Molecular Medicine Partnership Unit, University of Heidelberg and European Molecular Biology Laboratory, Heidelberg, Germany, Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Heidelberg, Germany, European Molecular Biology Laboratory, Heidelberg, Germany

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  • Styliani Lamprinaki,

    Affiliations: Molecular Medicine Partnership Unit, University of Heidelberg and European Molecular Biology Laboratory, Heidelberg, Germany, European Molecular Biology Laboratory, Heidelberg, Germany

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  • Matthias W. Hentze,

    Affiliations: Molecular Medicine Partnership Unit, University of Heidelberg and European Molecular Biology Laboratory, Heidelberg, Germany, European Molecular Biology Laboratory, Heidelberg, Germany

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  • Andreas E. Kulozik mail

    Niels.Gehring@med.uni-heidelberg.de (NHG); Andreas.Kulozik@med.uni-heidelberg.de (AEK)

    Affiliations: Molecular Medicine Partnership Unit, University of Heidelberg and European Molecular Biology Laboratory, Heidelberg, Germany, Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Heidelberg, Germany

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  • Published: May 26, 2009
  • DOI: 10.1371/journal.pbio.1000120

Reader Comments (1)

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key paper for everybody interested in EJC assembly and NMD

Posted by mueli on 19 Aug 2009 at 09:59 GMT

A remarkable paper. The high quality results of these elegant experiments allow absolutely compelling conclusions. The ordered splicing-dependent assembly of the eIF4A3-Y14-MAGOH pre-EJC followed by the splicing-independent association of BTZ and UPF3b has been elucidated and BTZ's role in NMD has been characterized.

No competing interests declared.