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Research Article

Targeting A20 Decreases Glioma Stem Cell Survival and Tumor Growth

  • Anita B. Hjelmeland mail,

    hjelmea@ccf.org (ABH); richj@ccf.org (JNR)

    Affiliation: Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, United States of America

    X
  • Qiulian Wu,

    Affiliation: Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, United States of America

    X
  • Sarah Wickman,

    Affiliation: Department of Surgery, Duke University Medical Center, Durham, North Carolina, United States of America

    X
  • Christine Eyler,

    Affiliations: Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, United States of America, Department of Surgery, Duke University Medical Center, Durham, North Carolina, United States of America

    X
  • John Heddleston,

    Affiliation: Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, United States of America

    X
  • Qing Shi,

    Affiliation: Department of Surgery, Duke University Medical Center, Durham, North Carolina, United States of America

    X
  • Justin D. Lathia,

    Affiliation: Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, United States of America

    X
  • Jennifer MacSwords,

    Affiliation: Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, United States of America

    X
  • Jeongwu Lee,

    Affiliation: Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, United States of America

    X
  • Roger E. McLendon,

    Affiliation: Department of Pathology, Duke University Medical Center, Durham, North Carolina, United States of America

    X
  • Jeremy N. Rich mail

    hjelmea@ccf.org (ABH); richj@ccf.org (JNR)

    Affiliation: Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, United States of America

    X
  • Published: February 23, 2010
  • DOI: 10.1371/journal.pbio.1000319

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Publisher's Note: Error in the Immunofluorescence Staining section

Posted by plosbiology on 04 May 2010 at 00:53 GMT

Cells, neurospheres, or tumor sections were fixed with 4% paraformaldehyde, washed with Tris-buffered saline, and incubated with polyclonal mouse anti-A20 (Santa Cruz Biotechnology) and goat anti-Sox2 (Santa Cruz Biotechnology) where indicated.
http://plosbiology.org/article/info:doi/10.1371/journal.pbio.1000319#article1.body1.sec4.sec4.p1

There was an error in the first sentence of the Immunofluorescence Staining section. The correct sentence is: Cells, neurospheres, or tumor sections were fixed with 4% paraformaldehyde, washed with Tris-buffered saline, and incubated with mouse anti-A20 (Santa Cruz Biotechnology) and rabbit anti-Sox2 (Santa Cruz Biotechnology) where indicated.

No competing interests declared.